Minicircle and Miniplasmid DNA Vectors

This first title on the topic provides complete coverage, including the molecular basis, production and possible biomedical applications. Written by the most prominent academic researchers in the field as well as by researchers at one of the world's leading companies in industrial production of minicircle DNA, this practical book is aimed at everyone who is directly or indirectly involved in the development of gene therapies.

Autorentext
Dr. Martin Schleef studied Biology at the Universities of Wurzburg and Bielefeld, Germany and holds a PhD from the University of Bielefeld.
Martin Schleef received post-doctoral training from the Institut Pasteur Paris, France. He joined QIAGEN GmbH, Hilden, Germany in 1994 and is co-founder and CEO of PlasmidFactory in Bielefeld since 2000.

Inhalt
The History of Minicircle (Martin Grund, Martin Schleef)
Operator-Repressor Titration: Stable Plasmid Maintenance without Selectable Marker Genes (Rocky M. Cranenburgh)
Selection by RNA/RNA Interaction: Maximally Minimized Antibiotic-Resistance Free Plasmids (Reingard Grabherr, Jurgen Mairhofer)
Plasmid-Based Medicinal Products -
Focus on pFAR: A Miniplasmid Free of Antibiotic Resistance Markers (Daniel Scherman, Corinne Marie, Mickael Quiviger, Helen Foster, George Dickson)
Plasmid DNA Concatemers -
Influence of Plasmid Structure on Transfection Efficiency (Christof Maucksch, Bronwen Connor, Carsten Rudolph)
Analytical Tools in Minicircle Production (Martin Schleef, Anja Rischmuller, Martina Viefhues, Mareike Dieding, Markus Blaesen, Marco Schmeer, Ruth Baier, Dario Anselmetti)
Utilising Minicircle Vectors for the Episomal Modification of Cells (Richard P. Harbottle, Orestis Argyros, Suet-Ping Wong, Charles Coutelle)
Replicating Minicircles: Overcoming the Limitations of Transient and of Stable Expression Systems (Jurgen Bode, Kristina Nehlsen, Sandra Broll, Raju Kandimalla, Niels Heinz, Markus Heine, Stefanie Binius, Axel Schambach)
Magnetofection of Minicircle DNA Vectors (Olivier Zelphati, Flavie Sicard, Cedric Sapet, Nicolas Laurent, Elodie Bertosio, Melanie Bertuzzi)
Minicircle Based Vectors for Non-Viral Gene Therapy: in vitro Characterization and in vivo Application (Dennis Kobelt, Jutta Aumann, Martin Schleef, Marco Schmeer, Ulrike Stein, Peter M. Schlag, Wolfgang Walther)
Episomal Expression of Minicircles and Conventional Plasmids in Mammalian Embryos (Wilfried A. Kues, Wiebke Garrels, Khursheed Iqbal)
Tissue Targeted Gene Electro-delivery of Minicircle DNA (Sophie Chabot, Muriel Golzio, Justin Teissie)
Increased Efficiency of Minicircles vs Plasmids under Gene Electrotransfer Suboptimal Conditions: An Influence of the Extracellular Matrix (V. Joubert, Franck M. Andre, Marco Schmeer, Martin Schleef, Lluis M. Mir)