MicroRNA Detection and Target Identification

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aimsto ensure successful results in the further study of this vital field.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt
Detection of miRNAs.- MicroRNA Identification, Target Prediction, and Validation for Crop Improvement.- MicroRNA Detection with CRISPR/Cas.- Detection of MicroRNAs by Northern Blot.- MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore.- Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor.- Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification.- Small RNA Profiling by Next Generation Sequencing Using High-Definition Adapters.- Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification.- Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine.- Determining miRNA Expression Patterns in Xenopus.- Overview of Computational and Experimental Methods to Identify Tissue-Specific MicroRNA Targets.- sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non-Model Species.- MicroRNA-Target Identification: A Combinatorial In Silico Approach.- An Efficient CRISPR-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis .- Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering.