Culture of Cells for Tissue Engineering

Step-by-step, practical guidance for the acquisition, manipulation,
and use of cell sources for tissue engineering

Tissue engineering is a multidisciplinary field incorporating
the principles of biology, chemistry, engineering, and medicine to
create biological substitutes of native tissues for scientific
research or clinical use. Specific applications of this technology
include studies of tissue development and function, investigating
drug response, and tissue repair and replacement. This area is
rapidly becoming one of the most promising treatment options for
patients suffering from tissue failure.

Written by leading experts in the field, Culture of Cells
for Tissue Engineering offers step-by-step, practical
guidance for the acquisition, manipulation, and use of cell sources
for tissue engineering. It offers a unique focus on tissue
engineering methods for cell sourcing and utilization, combining
theoretical overviews and detailed procedures.

Features of the text include:

• Easy-to-use format with a two-part organization

• Logically organized--part one discusses cell sourcing,
  preparation, and characterization and the second part examines
  specific engineered tissues

• Each chapter covers: structural and functional properties of
  tissues, methodological principles, culture, cell
  selection/expansion, cell modifications, cell seeding, tissue
  culture, analytical assays, and a detailed description of
  representative studies

• End-of-chapter features include useful listings of sources for
  reagents, materials, and supplies, with the contact details of the
  suppliers listed at the end of the book

• A section of elegant color plates to back up the figures in the
  chapters

Culture of Cells for Tissue Engineering gives
novice and seasoned researchers in tissue engineering an invaluable
resource. In addition, the text is suitable for professionals in
related research, particularly in those areas where cell and tissue
culture is a new or emerging tool.

Autorentext

GORDANA VUNJAK-NOVAKOVIC, PhD, is Professor in the
Department of Biomedical Engineering at Columbia University, New
York. She is the author of more than 125 papers and twenty-five
book chapters on biotechnology, biomechanics, orthopedics, and
tissue engineering.

R. IAN FRESHNEY, PhD, is Senior Research Fellow in the
Centre for Oncology and Applied Pharmacolgy at the University of
Glasgow. He is the author or editor of numerous books and a
world-renowned expert on cell culture technique.

Zusammenfassung
Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure.

Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures.

Features of the text include:

• Easy-to-use format with a two-part organization
• Logically organizedpart one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues
• Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
• End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book
• A section of elegant color plates to back up the figures in the chapters
  Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.

Inhalt

Preface vii

List of Abbreviations xi

Part I: Cell Culture

1. Basic Principles of Cell Culture 3
   R. Ian Freshney

2. Mesenchymal Stem Cells for Tissue Engineering 23
   Donald P. Lennon and Arnold I. Caplan

3. Human Embryonic Stem Cell Culture for Tissue Engineering 61
   Shulamit Levenberg, Ali Khademhosseini, Mara Macdonald, Jason Fuller, and Robert Langer

4. Cell Sources for Cartilage Tissue Engineering 83
   Brian Johnstone, Jung Yoo, and Matthew Stewart

5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering 113
   Henning Madry

Part II: Tissue Engineering

6. Tissue Engineering: Basic Considerations 131
   Gordana Vunjak-Novakovic

7. Tissue Engineering of Articular Cartilage 157
   Koichi Masuda and Robert L. Sah

8. Ligament Tissue Engineering 191
   Jingsong Chen, Jodie Moreau, Rebecca Horan, Adam Collette, Diah Bramano, Vladimir Volloch, John Richmond, Gordana Vunjak-Novakovic, David L. Kaplan, and Gregory H. Altman

9. Cellular Photoencapsulation in Hydrogels 213
   Jennifer Elisseeff, Melanie Ruffner, Tae-Gyun Kim, and Christopher Williams

10. Tissue Engineering Human Skeletal Muscle for Clinical Applications 239
    Janet Shansky, Paulette Ferland, Sharon McGuire, Courtney Powell, Michael DelTatto, Martin Nackman, James Hennessey, and Herman H. Vandenburgh

11. Engineered Heart Tissue 259
    Thomas Eschenhagen and Wolfgang H. Zimmermann

12. Tissue-Engineered Blood Vessels 293
    Rebecca Y. Klinger and Laura E. Niklason

13. Tissue Engineering of Bone 323
    Sandra Hofmann, David Kaplan, Gordana Vunjak-Novakovic, and Lorenz Meinel

14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering 375
    Peter I. Lelkes, Brian R. Unsworth, Samuel Saporta, Don F. Cameron, and Gianluca Gallo

15. Tissue Engineering of the Liver 417
    Gregory H. Underhill, Jennifer Felix, Jared W. Allen, Valerie Liu Tsang, Salman R. Khetani, and Sangeeta N. Bhatia

Suppliers List 473

Glossary 483

Index 491