Immunology: Overview and Laboratory Manual

A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.

Auteur

Tobili Sam-Yellowe is a research scientist and educator with over 30 years experience in malaria research, vaccine discovery, and research design and analysis. Dr. Sam-Yellowe is Professor of Biology at Cleveland State University.

Contenu

Part I: Overview of Immunity 1 Immunity 2 Components of immunity 3 Hematopoiesis4 The Immune system: pathogen sensing and detection 5 Lymphoid organs 6 Innate and Adaptive immunity 7 Leukocyte homing, migration and recirculation 8 Apoptosis 9 Antigens 10 B cell development, activation and immunoglobulin structure 11 Immunoglobulin and T cell receptor gene rearrangements12 Major histocompatibility complex proteins 13 T cell development and T cell receptor structure 14 Cytokines 15 Lymphocyte signals 16 Complement fixation 17 Cell mediated immunity 18 Hypersensitivities19 Parasite Immunity 20 Vaccines 21 Transplantation 22 Immune regulation and autoimmunity 23 Immunodeficiencies 24 Cancer 25 Practice questions 26 Study guide for exam 1 Part II: Laboratory Manual27. Immunology laboratory policies28. Instructions for notebook entries29. Instructions for writing lab reports 30. Lab report topicsEXERCISE 1: Using a compound microscopeEXERCISE 2: Pipetting and dilution technique, identification of mouse lymphoid organsEXERCISE 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing EXERCISE 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigensEXERCISE 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cellsEXERCISE 6: Clearance of bacteria from mouse blood by the RESEXERCISE 7: Hemolytic Plaque assay (The Jeme Plaque Assay)EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assaysEXERCISE 9: Determination of Antibody Titer: Screening TechniquesEXERCISE 10: Immunodiffusion and ImmunoelectrophoresisEXERCISE 11: Protein Affinity Purification of Rabbit IgGEXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)EXERCISE 13: Immunoblotting (Western Blotting)EXERCISE 14: Protein AssayEXERCISE 15: Enzyme-linked Immunosorbent Assay (ELISA)EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT)EXERCISE 17: Immunofluorescence assay (IFA) EXERCISE 18: Flow CytometryEXERCISE 19: Complement Fixation AssaysTissue Cell Culture TechniquesEXERCISE 20: Mitogen Induced Response of Lymphocytes:Detection of Band T cell Lymphocytes Using ImmunofluorescenceEXERCISE 21: Induction of IL-2 Secretion from Mouse Lymphoma CellsEXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody ProductionEXERCISE 23: Macrophage Migration InhibitionEXERCISE 24: Monoclonal Antibody PreparationEXERCISE 25: Immunization of mice with a recombinant proteinEXERCISE 26: Polarization of T helper cells into TH1 subsetEXERCISE 27: ImmunoprecipitationEXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markersEXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodiesAppendixSample Practice Questions