The Use Of Radioautography in Investigating Protein Synthesis

The Use of Radioautography in Investigating Protein Synthesis represents the 1965 Symposium on The Use of Radioautography in Investigating Protein Synthesis, supported by the International Society for Cell Biology.
This book is composed of four parts encompassing 18 chapters. The opening part deals first with the basic aspects and mode of operation of the radioautography technique. This part also examines the advantages and potentials of this technique compared to other cytological techniques. The next parts are devoted to the experimental methods of protein synthesis in nucleus and in cells of various types, as revealed by the electron microscope radioautography. The concluding part discusses the secretion and synthesis of protein enzymes by the acinar cells in rat pancreas and fibroblasts in wound healing. This part also considers the application of radioautography to determining the sites of synthesis of proteins.
This book is of great value to cell biologists, biochemists, cytologists, histologists, and pathologists.

Contenu

List of Contributors
Preface
Opening Address
Techniques and Methods
State of Light Autoradiography
Assessment of Technical Steps in Electron Microscope Autoradiography
Comparison of Emulsions and Techniques in Electron Microscope Radioautography
Application of Freeze-Drying and Formaldehyde-Vapor Fixation to Radioautographic Localization of Soluble Amino Acids. By
Protein Synthesis in Nucleus
Interchange of Protein between Nucleus and Cytoplasm
Amino Acid Deprivation and Deoxyribonucleic Acid Synthesis in Tetrahymena
Cytoplasmic Protein Synthesis in Cells of Various Types and Its Relation to Nuclear Protein Synthesis
Protein Synthesis in Cells of Various Types
Protein Synthesis in Lymphocytes
Fate of Newly Synthesized Proteins in Neurons
Protein Synthesis in the Ear under Normal and Pathological Conditions
Amino Acid Utilization in the Synthesis of Enamel and Dentin Matrices as Visualized by Autoradiography
Protein Synthesis and Cells of the Skeletal System
Synthesis and Turnover of Collagen in Periodontal Tissues
Secretory Processes
Secretion of Protein Enzymes by the Acinar Cells of the Rat Pancreas
Synthesis and Secretion of Collagen by Fibroblasts in Healing Wounds
The Formation of the Basement Lamella in Regenerating Salamander
The Elaboration of Thyroglobulin by the Thyroid Follicular Cells
What Radioautography Has Added to Protein Lore
Author Index