Techniques in Protein Chemistry IV

Techniques in Protein Chemistry IV compiles papers presented at the Sixth Protein Society Symposium held in San Diego, California in 1992.
This book discusses the mass spectrometry in protein sequence and structural investigations; site specific heterogeneity of N-linked oligosaccharides on recombinant human erythropoietin; and modification of thiophosphorylated proteins with extrinsic probes. The cysteine and tryptophan amino acid analysis of ABRF92-AAA; development of separation strategies for proteins by capillary electrophoresis; and peptide mapping of 2-D gel proteins by capillary HPLC are also elaborated. This text likewise covers the single syringe-pump solid-phase protein sequencer; hydrophobic contact density distribution functions; and application of chemical shift calculation to protein structure determination by NMR.
This publication is valuable to biologists and students concerned with the developments in mass spectrometry of proteins.

Contenu

Foreword

Preface

Acknowledgments

Section I Mass Spectrometry of Proteins and Peptides

Mass Spectrometry in Protein Sequence and Structural Investigations

Elucidation of Covalent Modifications and Noncovalent Associations in Proteins by Electrospray Ionization Mass Spectrometry

Identification of Conserved Protein Surface Metal-Binding Sites in Related Proteins by Mass Spectrometry

Matrix-Assisted Laser Desorption/ionization Mass Spectrometry of Membrane Proteins: The Scrapie Prion Protein

Characterization of the Proteins c-kit Ligand and DHFR by Electrospray Mass Spectrometry

Rapid Purification, Separation, and Identification of Proteins and Enzyme Digests Using Packed Capillary Perfusion Column LC and LC/MS

Sample Immobilization Protocols for Matrix Assisted Laser Desorption Mass Spectrometry

Assessing the Multimeric States of Proteins by Matrix-Assisted Laser Desorption Mass Spectrometry: Comparison with Other Biochemical Methods

N-Terminal Modification of Malarial Antigens from E. Coli

Mass Spectrometric Characterization of C-terminal "Ragged Ends" in Peanut Agglutinin

Accurate Determination of Molecular Mass for Proteins Isolated from Polyacrylamide Gels

Section II Analysis of Glycoproteins

Carbohydrate Characterization of a Glycoprotein by Matrix Assisted Laser Desorption Mass Spectrometry

Post-translational Modifications of Recombinant Proteins Determined by LC/Electrospray Mass Spectrometry and High Performance Tandem Mass Spectrometry

Identification and Characterization of Glycosylation Sites in Carcinoembryonic

Antigen (CEA) by Mass Spectrometry

Monosaccharide and Oligosaccharide Analysis of Recombinant Erythropoietin Electro-transferred onto Polyvinylidene Fluoride Membranes

A Practical Approach for Isolation and Characterization of Glycosylation Sites of Glycoproteins Bearing N- and/or 0-Linked Carbohydrate Chains

Location of the Keratan Sulfate Attachment Sites in the Hyaluronate Binding Region of the Proteoglycan, Aggrecan

Site Specific Heterogeneity of N-Linked Oligosaccharides on Recombinant Human Erythropoietin

Section III Phosphorylated Proteins

Analysis of Serine, Threonine, and Tyrosine Phosphorylation Sites with Mass Spectrometry

The Identification of Phosphorylation Sites in Large Membrane Proteins Following Their Isolation by SDS-PAGE

Modification of Thiophosphorylated Proteins with Extrinsic Probes

A Novel Method of Identifying Phosphorylation Sites Using a Thiophosphorylated Peptide and ESI-MS

Protein Phosphorylation: Sequence-Specific Identification of In Vivo Phosphorylation Sites by MALDI-TOF Mass Spectrometry

Synthetic Phosphopeptide Epitope Mapping with Mass Spectrometric Verification

Section IV Peptide Synthesis

Evaluation of Peptide Synthesis as Practiced in 53 Different Laboratories

Synthesis, Purification, and Interactions of Casein Signal Peptides

The Systematic Development of Peptides Having Potent Antimicrobial Activity Against E. coli through the Use of Synthetic Peptide Combinatorial Libraries

Synthesis of Proteins by Chemical Ligation of Unprotected Peptide Segments: Mirror-Image Enzyme Molecules, D- and L-HIV Protease Analogs

Difficult Sequences in the Synthesis of G-Protein Fragments That Harbor a Putative Adhesion Motif

Section V Amino Acid Analysis

Cysteine and Tryptophan Amino Acid Analysis of ABRF92-AAA

Compositional Protein Analysis Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl arbamate, a Novel Derivatization Reagent

Sensitive Analysis of Cystine/Cysteine using 6-Aminoquinolyl-NHydroxysuccinimidyl Carbamate (AQC) Derivatives

Amino Acid Analysis and Multiple Methylation of Lysine Residues in the Surface Protein Antigen of Rickettsia Prowazeki

Chirality Determination of D- and L- Amino Acids of Synthetic and Naturally Occurring Peptides

Section VI Protein Separations

Development of Separation Strategies for Proteins by Capillary Electrophoresis

High Resolution Full-Range (pl = 2.5 to 10.0) Isoelectric Focusing of Proteins and Peptides in Capillary Electrophoresis

Purification of Different Biologically Active Forms of Mouse ß-Nerve Growth Factor by Immobilized Metal Ion Affinity Chromatography

Increasing the Antigen Binding Capacity of Immobilized Antibodies

Section VII Peptide Mapping

Micropreparative Capillary Electrophoresis (MPCE) and Micropreparative HPLC of Protein Digests

Peptide Mapping of 2-D Gel Proteins by Capillary HPLC

Internal Peptide Sequence of Proteins Digested In-Gel after One- or Two- Dimensional Gel Electrophoresis

In Situ Tryptic Digestion of Proteins Separated by SDS-PAGE: Improved Procedures for Extraction of Peptides Prior to Microsequencing

In Situ Proteolytic Digestions Performed on Proteins Bound to the Hewlett-Packard Hydrophobic Sequencing Column

Section VIII Protein Sequencing

Routine Protein Sequence Analysis Below Ten Picomoles: One Sequencing Facility's Approach

Successful Peptide Sequencing with Femtomole Level PTH-Analysis: A Commentary

On-Line Microbore HPLC Detection of Femtomole Quantities of PTH-Amino Acids

Single Syringe-Pump Solid-Phase Protein Sequencer

Analysis of Complex Protein Mixtures on the HP-GIOOOA Sequencer

Protein Sequencing of Post-Translationally Modified Peptides and Proteins: Design, Characterization and Results of ABRF-92SEQ

Carboxy-terminal Protein Sequence Analysis Using Carboxypeptidase and Electrospray Mass Spectrometry

Protein Ladder Sequencing: A Conceptually Novel Approach to Protein Sequencing Using Cycling Chemical Degradation and One-Step Readout by Matrix-Assisted Laser Desorption Mass Spectrometry

Section IX Protein Conformation, Folding, and Modeling

The Use of Molecular Modeling to Delineate B-Cell and T-Cell Epitopes of Human Sperm-Specific LDH-C4

Hydrophobic Contact Density Distribution Functions

Construction and Functional Selection of a T4 Lysozyme Gene Library Randomly Mutagenized at Five Specific Sites

Horse Heart Ferricytochrome c: Anions, Buffers, and Stability

Measurement of Amide Hydrogen D/H Franctionation Factors in Proteins by NMR Spectroscopy

A Model for the Molten Globule State of CTF Generated Using Molecular Dynamics

Urea and Guanidine-HCl Yield Different Unfolding Free Energies for CheY: Which Denaturant Provides the Most Reliable Free Energy Values?

Learning an Objective Alphabet of Amino Acid Conformation in Protein

AAG°(WT-mut) for BPTI Hydrophobic Core Mutants Measured by Hydrogen Isotope Exchange

Thermodynamics of Side Chain Internal Rotations - Effects on Protein Structure and Stability

Section X NMR Analysis of Protein and Peptide Structure

Evaluation of NMR Based Structure Determination of Flexible Peptides: …