Synthetic Biology

Synthetic Biology provides a framework to examine key enabling components in the emerging area of synthetic biology. Chapters contributed by leaders in the field address tools and methodologies developed for engineering biological systems at many levels, including molecular, pathway, network, whole cell, and multi-cell levels. The book highlights exciting practical applications of synthetic biology such as microbial production of biofuels and drugs, artificial cells, synthetic viruses, and artificial photosynthesis. The roles of computers and computational design are discussed, as well as future prospects in the field, including cell-free synthetic biology and engineering synthetic ecosystems.

Synthetic biology is the design and construction of new biological entities, such as enzymes, genetic circuits, and cells, or the redesign of existing biological systems. It builds on the advances in molecular, cell, and systems biology and seeks to transform biology in the same way that synthesis transformed chemistry and integrated circuit design transformed computing. The element that distinguishes synthetic biology from traditional molecular and cellular biology is the focus on the design and construction of core components that can be modeled, understood, and tuned to meet specific performance criteria and the assembly of these smaller parts and devices into larger integrated systems that solve specific biotechnology problems.

• Includes contributions from leaders in the field presents examples of ambitious synthetic biology efforts including creation of artificial cells from scratch, cell-free synthesis of chemicals, fuels, and proteins, engineering of artificial photosynthesis for biofuels production, and creation of unnatural living organisms
• Describes the latest state-of-the-art tools developed for low-cost synthesis of ever-increasing sizes of DNA and efficient modification of proteins, pathways, and genomes
• Highlights key technologies for analyzing biological systems at the genomic, proteomic, and metabolomic levels which are especially valuable in pathway, whole cell, and multi-cell applications
• Details mathematical modeling tools and computational tools which can dramatically increase the speed of the design process as well as reduce the cost of development.

Contenu

Introduction Section I. Synthesis and engineering tools in synthetic biology Chapter 1. DNA synthesis Chapter 2. Protein engineering Chapter 3. Pathway engineering Chapter 4. Biological standardization

Section II. Computational and theoretical tools in synthetic biology Chapter 5. Biocomplexity issues in synthetic biology Chapter 6. Computational protein design Chapter 7. Computer-Aided Design in Synthetic Biology Chapter 8. Computational tools for strain design

Sections III. Applications in synthetic biology Chapter 9. Pathway/network level: design and applications of therapeutic genetic circuits Chapter 10. Pathway/network level: production of drugs Chapter 11. Pathway/network level: synthesis of new fuels Chapter 12. Organism level: genome assembly Chapter 13. Multi-cell level: synthetic prey-predator system

Section IV. Future prospects Chapter 14. Design of artificial cells Chapter 15. Cell-free synthetic biology Chapter 16. Artificial photosynthesis Chapter 17. Engineering synthetic ecosystems