Structure-Function Relationships of Proteolytic Enzymes

Structure-Function Relationships of Proteolytic Enzymes provides information pertinent to the fundamental aspects of proteolytic enzymes. This book presents the historical role of proteolytic enzyme as a group in protein and enzyme chemistry.

Organized into 23 chapters, this book begins with an overview of the results obtained from investigation on the chymotrypsinogens of porcine origin. This text then examines the differences of amino acid sequence between chymotrypsin, trypsin, and elastase that affect the substrate binding site, which reflect the specificity differences between these enzymes. Other chapters consider the kinetic parameters related to the trypsin-catalyzed hydrolysis of several model peptides. This book discusses as well the acetylation of trypsin, which result in functional consequences varying from complete inactivation to promotion of activity. The final chapter deals with the physical properties of stem bromelain in comparison with the data for three other sulfhydryl proteases of plant origin.

This book is a valuable resource for enzymologists, microbiologists, and biochemists.

Contenu

List of Participants

Foreword

Session I: Pancreatic Proteases

On the Three Chymotrypsinogens of Porcine Pancreas

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Chymotrypsin: Tertiary Structure and Enzymatic Activity (Abstract)

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The Role of Carboxylates and Phenol Side-Chains in the Activity and Conformation of Trypsinogen, Trypsin, Chymotrypsinogen, and Chymotrypsin

Chemical Modifications of Bovine Trypsinogen and Trypsin

The Specificity Site of Trypsin

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Structure and Mechanism of Action of a Pancreatic Trypsin Inhibitor

The Chemistry of the Reactive Site of Soybean Trypsin Inhibitor

Structural Aspects of Interaction of Trypsin with Macromolecular Inhibitors

Homology and Phylogeny of Proteolytic Enzymes

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Structural Aspects of Thrombin and Prothrombin

Cobalt Proteases: Implications of Absorption and Circular Dichroism Spectra for Catalysis

Session II: Microbial Proteases

Some Structure-Function Relationships in the Subtilisins

Subtilisin BPN': Tertiary Structure and Inhibitor Binding (Abstract)

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Subtilisin: Stability Properties and Secondary Binding Sites

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Aminopeptidases from Thermophilic Microorganisms

The Second Kaj Linderstrom-Lang Gold Medal Lecture

A Synthetic Approach to the Study of Microenvironmental Effects on Enzyme Action

Session III: Pepsin and Related Enzymes

Specificity and Mechanism of Pepsin Action

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Towards a Mechanism for Pepsin

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Chemical Studies on Purified Pepsin

Porcine Pepsinogen and Pepsin

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Session IV: Sulfhydryl Proteases

The Three-Dimensional Structure of Papain

Streptococcal Proteinase

Structure and Function of Stem Bromelain