Standard Methods of Clinical Chemistry

Standard Methods of Clinical Chemistry, Volume 6 provides information pertinent to the more accurate methods of analysis. This book deals with various subjects, including personnel management, electronics, and data processing systems.

Organized into 21 chapters, this volume begins with an overview of the most colorimetric methods for estimating uric acid based on the nonspecific reduction of phosphotungstate by uric acid in an alkaline solution. This text then examines the electrophoretic separation and quantitation of proteins in serum or other body fluids. Other chapters provide a discussion of the control of the major reaction variables needed to meet the recommendations of the International Union of Biochemistry. This book discusses as well the modifications developed to eliminate some of the inaccuracies resulting from incomplete destruction of hydrogen peroxide and instability of the developed chromophore. The final chapter deals with the enzymatic methods for the determination of lactic and pyruvic acids in body fluids and tissues.

This book is a valuable resource for clinical chemists.

Contenu

Harry H. Sobotka-A Biographical Sketch
Contributors
Preface
Uric Acid (Uricase)
Cellulose Acetate Electrophoresis of Protiens of Serum, Cerebrospinal Fluid, and Urine
The International Unit: Standardization of Enzyme Activity Units
Hydroxyproline in Urine and Tissues
Porphyrins, Porphobilinogen, and Aminolevulinic Acid in Urine
Toxicology in a General Hospital
Spectrophotometric Quantitation of Plasma and Urinary Amino Nitrogen with Fluorodinitrobenzene
Colorimetric Determination of Vanilmandelic Acid in Urine
Hemoglobin in Plasma
Ion-Exchange Method for the Determination of Plasma Ammonia Nitrogen with the Berthelot Reaction
Electrophoretic Determination of Serum Lipoproteins (Prestaining Technique)
The Determination of Total Seromucoid (as Hexose)
Glatamic Oxalacetic Transaminase (Colorimetric)
The Determination of Glocuse by the Ortho-Toluidine Method (Filtrate and Direct Procedure)
Oxygen, Partial Pressure (PO2) Electrode Method
Iodometric Measurement of Amylase (Caraway)
Serum Lithium Determination by Atomic Absorption Spectroscopy
Calculi
Calcium (Fluorometric)
Triglycerides ("Neutral Fat") in Serum
Nomenclature for Quantities and Units
Enzymatic Methods for Lactic and Pyruvic Acids
Author Index 261
Subject Index