Research on Steroids

Research on Steroids contains the proceedings of the Fourth Meeting of the International Study Group for Steroid Hormones. The papers explore the production, chemical characterization, and metabolism of steroid hormones and their interactions with proteins. Topics range from the action of estradiol in vitro to water-soluble metabolites of estrogens, transport of steroids by proteins, and immunological aspects of steroid hormones. The binding of steroids by tissue proteins is also discussed, along with the use of specific plasma proteins for the determination of steroids.
This volume is comprised of 38 chapters and begins with an overview of the general aspects of steroid-protein interaction, followed by a discussion on quantitative studies of steroid binding proteins; aromatization of testosterone by human placenta enzymes; relationship between plasma binding and 5a-reduction of testosterone; cortisol-binding capacity of plasma transcortin in subjects treated with various anabolic steroids; and plasma testosterone binding capacity and estrogen excretion in normal and pathological pregnancies. Subsequent chapters deal with the interaction between estrogenic and carcinogenic substances in the rat mammary gland; influence of steroid production on serum albumin in Cushing's syndrome; and nuclear estradiol binding proteins in the uterus.
This book will be of interest to biochemists, biologists, and physiologists.

Contenu

Section I General Aspects of Steroid-Protein Interaction

Lecture

General Aspects of Steroid-Protein Interaction

Communications

The "Biological Exchange Constant" of Estradiol-17ß between Plasma and Uterus in Adult and Immature Rats

Quantitative Studies of Steroid Binding Proteins

Action of Oestradiol In Vitro: Increase in Ribonucleic Acid Biosynthesis in Uterine Nuclei

Aromatization of Testosterone by Human Placenta Enzymes: Role of a Protein Fraction obtained from pregnant Female Serum which binds Testosterone (T) and Oestradiol-17ß(E2)

Stability and Nature of In Vivo Binding between Testosterone, its Metabolites and Organ Proteins in Rats

Water Soluble Metabolites of Oestrogens: A Model of Covalent Steroid Protein Binding

Section 2 Transport of Steroids by Proteins

Lecture

Transport of Steroids by Proteins

Communications

The Binding of Progesterone by the a1-Acid Glycoprotein

Interaction of Steroids with Human Red Blood Cells

Relationship between Plasma Binding and 5a-Reduction of Testosterone

Comparative Studies on the Binding of Testosterone and 17ß-Oestradiol by Serum Proteins in Normals and in Patients with Liver Cirrhosis

Cortisol-Binding Capacity of Plasma Transcortin in Subjects Treated with Various Anatolic Steroids

Section 3

Communications

A 4S Oestradiol-Binding Protein in Uteri from Mature Rats that is Controlled by Endogenous Hormones

Plasma Testosterone Binding Capacity and Oestrogen Excretion in Normal and Pathological Pregnancies

Interaction between Oestrogenic and Carcinogenic Substances in the Rat Mammary Gland

Metabolization and Binding of Oestradiol-17ß by Rat Ventral Prostate In Vitro

Interaction of Diethylstilbestrol with Uterine Receptor Sites

Biological Inactivation of Progesterone by Interaction with Corticosteroid-Binding Globulin and with Albumin

Effects of Clomiphene Citrate on Binding Affinity of Testosterone, Oestradiol and Cortisol in Serum of Normal Adult Men

Influence of Steroid Production on Serum Albumin in Cushing's Syndrome

Section 4 Immunological Aspects of Steroid Hormones

Lecture

Studies with Antibodies to Steroids

Communications

Neutralization of Exogenous Estradiol Activity following Active Immunization Procedures

Antibodies to Oestrogens

Solid Phase Radioimmunoassay of Estradiol-17ß and Estrone

Section 5 Binding of Steroids by Tissue Proteins

Lecture

Binding of Steroids by Tissue Proteins. Steroid hormone, "Receptors"

Communications

Cortisol-Binding Protein from various Tissues

Progesterone-Binding by the Soluble Fraction of Corpus Luteum from the Pregnant Cow

Effect of I2 and Sulphydryl Reagents on Binding Activity of Estrogen Receptors

Nuclear Oestradiol Binding Proteins in the Uterus

Oestradiol-Binding and Protein Phosphorylation

Partial Purification of an Estrophilic Protein from Calf Uterus

Section 6 Use of Specific Plasma Proteins for the Determination of Steroids

Lecture

Use of Specific Plasma Proteins for the Determination of Steroids

Communications

Plasma Estrogen Assays Utilizing the Cytoplasmic Estrogen Binder of Rabbit Uterus

Plasma Progesterone and 17a-hydroxyprogesterone Measured by a Competitive Protein Binding Technique and Related to the Oestrogen Excretion and LH Level around Ovulation in the Normal Menstrual Cycle

ACTH-Induced Fall in Plasma Testosterone

A Method for Estimation of Plasma Testosterone using a Technique Depending on Competitive Protein Binding

Study of C19O2 Steroids in Human Plasma by Competitive Protein Binding

Partial Purification and Preliminary Characterization of an Estrogen Binding Protein (Estrogen Receptor) from Calf Uterus Nuclear Fraction

Author Index