In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included.

The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully.The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.

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Extraction of Total RNA from Tissues and Cultured Cells, Sandeep Raha, Mingfu Ling, and Frank Merante. Isolation of Total Cellular DNA from Tissues and Cultured Cells,Frank Merante, Sandeep Raha, and Mingfu Ling. Gel Electrophoresis of DNA, Duncan R. Smith. S1 Nuclease Analysis of RNA, Louis Lefebvre and Stéphane Viville. Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA), Ralf Einspanier and Annette Plath. Gene Probes, Marilena Aquino de Muro. Nonradioactive Labeling of DNA,Thomas P. McCreery and Terrence R. Barrette. Southern Blot Analysis, Paolo A. Sabelli . Northern Blot Analysis, Paolo A. Sabelli . DNA Sequencing, Chris Spencer . Autoradiography and Fluorography, Bronwen Harvey . Mobility Shift Assays, Nigel J. Savery and Stephen J. W. Busby. cDNA Libraries, Ian G. Cowell . 4 Genomic DNA Libraries, Bimal D. M. Theophilus . l as a Cloning Vector, Rupert Mutzel . Plasmid-Derived Cloning Vectors, Craig Winstanley and Ralph Rapley. M13 and Phagemid-Based Cloning Vectors. Ralph Rapley. Retroviral Vectors: From Laboratory Tools to Molecular Medicines. Richard G. Vile, Anna Tuszynski, and Simon Castleden. Baculovirus Vectors, Azeem Ansari and Vincent C. Emery. Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes. M. Alexandra Aitken, Selina Raguz, and Michael Antoniou. Plant Transformation, Andy Prescott, Rob Briddon, and Wendy Harwood. Restriction Fragment-Length Ploymorphisms,Elaine K. Green. Genome Mapping, Jacqueline Boultwood. Yeast Artificial Chromosomes,Angela Flannery and Rakesh Anand . Polymerase Chain Reaction, Ralph Rapley. In Vitro Transcription, Martin J. Tymms . In Vitro Translation, Martin J. Tymms. Site-Directed Mutagenesis, John R. Adair and T. Paul Wallace. Transgenic Techniques, Roberta M. James and Paul Dickinson . DNA Profiling: Theory and Practice, Karen M. Sullivan . Radiolabeling of Peptides and Proteins, Arvind C. Patel and Stewart R. Matthewson . Protein Electrophoresis,Paul Richards . Free Zone Capillary Electrophoresis, David J. Begley .Protein Blotting: Principles and Applications, Peter R. Shewry and Roger J. Fido . Ion-Exchange Chromatography, David Sheehan and Richard Fitzgerald . Size-Exclusion Chromatography, Paul Cutler . Hydrophobic Interaction Chromatography, Paul A. O'Farrell . Affinity Chromatography, George W. Jack. Reversed-Phase HPLC, Bill Neville . Glycoprotein Analysis, Terry D. Butters. Protein Sequencing, Bryan J. Smith and John R. Chapman. Solid-Phase Peptide Synthesis, Gregg B. Fields. Protein Engineering, Sudhir Paul . Monoclonal Antibodies, Christopher Dean and Helmout Modjtahedi . Phage-Display Libraries, Julia E. Thompson and Andrew J. Williams . Enzyme-Linked Immunosorbent Assay (ELISA), John R. Crowther. Epitope Mapping: Identification of Antibody Binding Sites on Protein Antigens, Glenn E. Morris. Immunocytochemistry, Lorette C. Javois .Flow Cytometry, Robert E. Cunningham. Mass Spectrometry, John R. Chapman . The Technique of In Situ Hybridization: Principles and Applications, Desiré du Sart and K. H. Andy Choo. Index.