Membrane Glycoproteins

Membrane Glycoproteins: A Review of Structure and Function deals with membrane glycoproteins found in biological systems. The book describes the structure and biosynthesis of the glycoproteins in relation to known or postulated functions in membranes.
The text opens with an introduction and a topic on detection and distribution of membrane glycoproteins. The book then notes that the isolation of membrane glycoproteins brought by the progress in research and technology of membrane solubilization and purification of the soluble components is now possible. Discussion is also directed to glycoproteins as being integral components of intracellular membranes, and not just located on cell surfaces. Through the structural analysis of glycoproteins produced by the secretory glands, analysis of human blood group antigens is available. Likewise, discoveries are made, explaining that lectins are useful reagents in detecting the type and numbers of glycoproteins found on cellular members. Lectins are likewise being widely used in tests for carbohydrate-containing substances in membrane-mediated processes. The metabolism, growth control, and cell surface reactions of membrane glycoproteins are also explained.
The book can serve as a guide for biologists, chemists, biochemists, and academicians interested in the study of membranes or glycoproteins.

Contenu

1. Introduction: Membranes and Membrane Glycoproteins
   
   2. Detection and Distribution of Membrane Glycoproteins
      Histochemical Evidence
      Histochemistry of Isolated Membranes
      Chemical Evidence
      Cell Coats, Glycocalyx, 'Fuzz' and Membrane Glycoproteins
      Molecular Dimensions of Membrane Glycoproteins
   3. Isolation of Membrane Glycoproteins
      Introduction
      Solubilisation Methods
      Separation and Purification Methods
      Polyacrylamide Gel Electrophoresis of Membrane Glycoproteins
      Erythrocyte Membranes
      KB Cell Membranes
   4. Structure of Membrane Glycoproteins
      A Model
      Erythrocyte Membrane Glycoproteins
      Cytochrome B5 and Cytochrome B5 Reductase
      Summary and Additional Evidence
      Surface Labelling of Membrane Glycoproteins
   5. Intracellular Membrane Glycoproteins
      Introduction
      Sarcoplasmic Reticulum
      Lysosomes
      Mitochondria
      Nuclei
      Zymogen Granules
      Milk Fat Globule Membrane
   6. Membrane Glycoproteins as Antigens
      Antigens of The ABO System
      Antigens of The MN System
      Strong Histocompatibility Antigens
   7. Lectins
      Properties of Lectins
      Glycoprotein Receptors For Lectins
      Lectin Cytotoxicity and Selection of Resistant Cells
      Reaction of Lectins With Cells
      Lectins as Histochemical Reagents
   8. Lymphocyte Membrane Glycoproteins
      Lymphoid Cells
      Lymphocyte Membranes
      Clusters and 'Cap' Formation
      Biological Implications In Lymphocyte Transformation
      Transformation of Lymphocytes Induced By Oxidation of Membrane Glycoproteins
   9. The Mobility of Membrane Glycoproteins
      Membrane Fluidity-A General Property of Cells
      Membrane-Intercalated Particles
      Cell Agglutination
   10. Biosynthesis of Glycoproteins
       Intermediary Metabolism and Sugar Labelling In Whole Cells
       Glycosyl Transferases
       Assembly of Carbohydrate Chains
       Membrane Flow In Glycoprotein Biosynthesis
       Fidelity of Glycoprotein Biosynthesis
       Lipid Intermediates In Glycoprotein Biosynthesis
       Control of Glycosyl Transferases
   11. Metabolism of Membrane Glycoproteins
       Biosynthesis of Membrane Glycoproteins and Membrane Biogenesis
       Surface Membrane Turnover
   12. Membrane Glycoproteins and Growth Control
       Cellular Regulation of Membrane Glycoprotein Biosynthesis
       Regulation of Membrane Glycoprotein Biosynthesis In Neoplastic Cells
       Membrane Glycoproteins In Cells With Finite Life Span In Culture
   13. Membrane Glycoproteins and Cell Surface Reactions
       Surface Located Enzymes of Glycoprotein Metabolism
       Roles In Adhesion
       Roles In Glycoprotein Catabolism
       Glycoprotein Structure and The Specificity of Synaptic Connections
       Appendix A: Terminal Sugar Sequences of Membrane Glycoproteins In Nervous Tissue
       Appendix B: Glycoprotein Components of Myelin
       References
       Index