Protocols for Neural Cell Culture

In Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures.
Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.

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The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora­ tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.

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In Vitro Assays for Axonal Growth and Targeting Valérie Castellani and Jürgen Bolz Interface Organotypic Hippocampal Slice Cultures Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, and Luc Stoppini Slice Cultures for Study of Microglia Carol A. Colton, Meggan Czapiga, and Toby N. Behar Microexplant Cultures of the Cerebellum Bernard Rogister and Gustave Moonen Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons Robert B. Campenot and Grace Martin Chick Spinal Somatic Motoneurons in Culture Bernhard H. J. Juurlink Primary Cultures of Sympathetic Ganglia Mary I. Johnson Primary Cell Cultures for the Study of Myelination Mary I. Johnson, Richard P. Bunge, and Patrick M. Wood Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures Ruth Cole and Jean de Vellis Culture of Glial Cells from Human Brain Biopsies V. Wee Yong and Jack P. Antel Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium Sergey Fedoroff and Arleen Richardson Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium Bernhard H. J. Juurlink, Shawn K. Thorburne, and Richard M. Devon Olfactory Ensheathing Cell Cultures Ronald Doucette Cultures of Stem Cells of the Central Nervous System Angela Gritti, Rossella Galli, and Angelo Luigi Vescovi Aggregating Neural Cell Cultures Paul Honegger and Florianne Monnet-Tschudi Neural Cell Lines Kaoru Murayama, Natalia N. Singh, Angela Helmrich, and David W. Barnes Virus Vectors for Gene Therapy of the Nervous System Sonia N. Yeung and Frank Tufaro Preparation of Substrata for In Vitro Culture of Neurons Paul C. Letourneau Serum-Fee Media for NeuralCell Cultures: Adult and Embryonic Paul J. Price and Gregory J. Brewer Development of Multiple-Well Plate Biological Assays John W. Harbell Neural Cell Counting: Cell Number Determination in Microplate Cultures Marston Manthorpe Assays for Neuronal Cell Migration Toby N. Behar Colony Culture: Plating Efficiency Assay and Cloning Sergey Fedoroff and Arleen Richardson Hybridoma Cultures for Production of Antibodies: Purification and Specificity of Antibodies J. Denry Sato Elimination of Cell Types from Mixed Neural Cell Cultures Richard M. Devon Quantification of Cells in Culture Arleen Richardson and Sergey Fedoroff Tips for Tissue Culture Arleen Richardson and Sergey Fedoroff