Protochlorophyllide Reduction and Greening

Proceedings of a Meeting held at the University of Liège, Belgium, 8-9 August 1983

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Proceedings of a Meeting held at the University of Liège, Belgium, 8-9 August 1983

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Preface.- Section 1: Protochlorophyllide Photooxidoreductase Properties and Localisation.- A critical appraisal of the role and regulation of NADPH-protochlorophyllide oxidoreductase in greening plants.- Changes in the properties of protochlorophyllide reductase during early greening of etiolated squash cotyledons.- Proteolytic effects on the protochlorophyllide-oxidoreductase in oat.- Characterization of PChlide-protein complexes in PT and PLB enriched fractions of bean etioplasts. Effects of NADPH.- Protochlorophyll (ide) and chlorophyll (ide) in isolated original and reformed prolamellar bodies.- Section 2: Intermediates in Protochlorophyllide Photo-Reduction.- The long-wavelength-absorbing quenchers formed during illumination of protochlorophyllide-proteins.- Early processes of protochlorophyllide photoreduction as measured by nanosecond and picosecond spectrophotometry.- The primary reaction in the photoreduction of protochlorophyllide. A nanosecond fluorescence study.- Section 3: Protochlorophyllide and Chlorophyllide forms.- Protochlorophyllide and chlorophyllide-protein complexes during greening.- Fluorescence emission spectra of etiolated leaves measured at 296 and 77K during the first seconds of continuous illumination.- The regulatory effect of the cysteine/cystine redox couple on protochlorophyllide photoreduction in etioplasts.- The influence of the NADPH/NADP+ redox couple on the photoreduction of protochlorophyllide and on the spectral characteristics of the chlorophyllide-proteins in etioplasts.- The photoreduction of Zinc-protochlorophyllide by isolated etioplast lamellae.- A model for the reduction of protochlorophyll (ide) containing three different forms of photoactive protochlorophyll (ide).- Section 4: The First Photoactivities Apart from Protochlorophyllide Photoreduction.- The Franck-Inoue chlorophyllide micro-cycle II in vivo and in vitro.- Extension of the experiment by J.H.C. Smith on the onset of the oxygen evolution in etiolated bean leaves.- Thermoluminescence in etiolated bean leaves.- Section 5: Membrane Development and Pigment Composition.- Biosynthesis of the pigment-protein complexes.- Low temperature fluorescence spectra of Phaseolus vulgaris plastids durinq development in periodic or continuous light in the presence of the internal fluorescence standard Phycocyanin.- The composition of stroma and grana thylakoid pigment-protein complexes.- Effect of light and protein crosslinkers on the ultrastructure of isolated etioplast membranes.- Section 6: Energy Migration.- The energy migration in pigment assembly in relation to the chlorophyll biosynthesis.- The bioenergetic description of light energy migration in photoactive membranes; equivalence between the theory of the energy fluxes and the theory of the proportion of pigment forms to total pigments.- The dynamics of the photoreduction of protochlorophyll (ide) into chlorophyll (ide).- Section 7: Models.- Photochemical reduction of protochlorophyllide in detergent micelles with the final chlorophyllide formation.- Molecular structure of protochlorophyll forms.- Section 8: Special Topics.- Dark synthesis of chlorophyll in vivo and dark reduction of protochlorophyllide in vitro by pea chloroplasts.- Effect of leaf age on protochlorophyllide and chlorophyllide formation (a review).- Author index.