Protides of the Biological Fluids

Protides of the Biological Fluids contains the proceedings of the 23rd Colloquium on "Protides of the Biological Fluids" held in Brugge, Belgium in 1975. The discussions were organized around three themes: proteinase inhibitors, clinical evaluation of plasma protein patterns, and affinity chromatography.

Comprised of 117 chapters, this volume begins with an overview of carcinofetal proteins, followed by an analysis of proteinase inhibition. The reader is then introduced to the mechanism and structure of human alpha-1-proteinase inhibitor; some properties of a complex between ?2-macroglobulin and brinase; and the interaction of proteinase inhibitors with blastocyst proteinases involved in implantation. Subsequent chapters deal with the thermodynamics and kinetics of the interaction of trypsin with trypsin inhibitors; purification and characterization of acid-stable proteinase inhibitors from human seminal plasma; and the possible role of protease inhibitors in cartilage metabolism. The book also explores the structure and function of animal proteinase inhibitors as well as techniques and methods used in clinical evaluation of plasma protein patterns.

This monograph will be of interest to biologists and research workers in biology.

Contenu

Preface

Acknowledgements

The Fourth Arne Tiselius Memorial Lecture

Carcinofetal Proteins

Section A. Proteinase Inhibitors

A1. Proteinase Inhibitor Interaction

A1.1. Molecular Aspects and Quantitation

Chemical Studies on Proteinase Inhibition

Mechanism and Structure of Human Alpha-1-Proteinase Inhibitor

Inhibition of Urokinase by Purified Human a1-Antitrypsin

Interaction Between Alpha1-Antitrypsin and Alpha2-Macroglobulin in the Elimination of Proteolytic Enzymes

Some Properties of a Complex Between a2-Macroglobulin and Brinase

Kunitz Bovine Trypsin Inhibitor: Interaction with a-Chymotrypsin

Kinetics of the Interaction of Virgin and Modified Bovine Trypsin Kallikrein Inhibitor (Kunitz) with ß-Trypsin

Interaction of Proteinase Inhibitors with Blastocyst Proteinases involved in Implantation

Blocking of C1 Esterase Inhibitor by Cold Promoted Activation of Factor VII

Direct Identification of Specific Protease Inhibitors in Mixtures by Modified Quantitative Immunoelectrophoresis

Studies on the Inhibition of Renin by Radioimmunoassay

A1.2. Kinetics and Thermodynamics

Thermodynamics and Kinetics of the Interaction of Trypsin with Trypsin Inhibitors

The Inhibition of Human and Bovine Trypsins and Chymotrypsins by the Inter-a-Inhibitor from Human Plasma

Kinetic and Thermodynamic Studies of Human Alpha-1-Proteinase Inhibitor

A2. Structure and Function of Human Proteinase Inhibitors

A2.1. In Plasma

Structure of a1-Antitrypsins

a-1-Proteinase Inhibitor Deficiency Disease: A Probable Inborn Error of Carbohydrate Side Chain Synthesis

New Data Concerning Inter-Alpha-Trypsin-Inhibitor

The Role of Sialic Acid in Hepatic Uptake of Alpha1-Antitrypsin

Preparative Separation of Various Bands of Alpha-1-Antitrypsin by Electrofocusing in Thin-layer Polyacrylamide Gel

Circular Dichroism Studies on a-1-Proteinase Inhibitor

Subunit Structure of Human Alpha-2-Macroglobulin. Its Relationships with its Biological Activity

Comparative Study of Rat c^-Macroglobulin and Rat a2-Macroglobulin: Interaction with Trypsin and Chymotrypsin

Primary Structure of Hirudin, A Thrombin-Specific Inhibitor

The Partial Purification and Characterization of Inter-a-Antiplasmin from Human Serum

A Low Molecular Weight Elastase Inhibitor from Human Serum

A2.2. In the Reproductory System

Acid-stable Proteinase Inhibitors from Human Seminal Plasma: Purification and Characterization

Proteinase Inhibitors in Testicular and Epididymal Fluid

An Acid-stable Proteinase Inhibitor in Human Cervical Mucus

A2.3. In Pancreatic Tissue

Human Pancreatic Secretory Trypsin Inhibitor, Amino Acid Sequence

1H NMR Studies of the Dynamics of the Solution Conformation of the Basic Pancreatic Trypsin Inhibitor (BPTI)

The Effect of Trypsin-Inhibitors on the Rat Exocrine Pancreas

Comparative 1H NMR Studies of the Solution Conformation of the Cow Colostrum Trypsin Inhibitor (CTI), The Trypsin Inhibitor of Helix Pomatia (HPI) and the Basic Pancreatic Trypsin Inhibitor (BPTI)

Isolation and Properties of an Aortic Elastase

Measurement of the Proteinase Inhibitors of the Bovine Pancreas by Radio-Immunoassay

A2.4. In Other Tissues

Tissue Inhibitors are Cell Growth Regulators

The Possible Role of Protease Inhibitors in Cartilage Metabolism

The Isolation, Purification and Partial Characterization of Proteinase Inhibitors from Bovine Cartilage and Aorta

Characterization of Proteinase-Inhibitor Complexes in the Purulent Secretions of Mucous Membranes as Complexes Between Leucocytic Elastase and Proteinase Inhibitors

A3. Structure and Function of Animal Proteinase Inhibitors

Evolution of Proteinase Inhibitors

Electrophoretic Variants of Serum a1-Antitrypsin in Baboons

Antitrypsin Polymorphism in Sub-Human Primates

Structure, Biochemistry and Comparative Aspects of Mammalian Seminal Plasma Acrosin Inhibitors

About the Aminoacid Sequence of the Proteinase Inhibitors from Dog Submandibularis Glands

Characterization and Inhibitory Properties of Snail Secretory Proteinase Inhibitors

Interaction of Exopeptidases with Specific Proteins from Ascaris Lumbricoides

Broad Specificity Proteinase Inhibitors from Sea Anemones

Section B. Clinical Evaluation Of Plasma Protein Patterns

B1. Techniques and Methods

An Automated Approach to the Preparation of Plasma Protein Profiles

Determination of Proteins by Laser Nephelometry

Immunochemical Determination of Blood Proteins

A New Radioimmunoassay of a-Fetoprotein and Carcinoembryonic Antigen

B2. Interpretation

Human Plasma Proteins Discovered During the Last Few Years and their possible Value for Clinical Diagnosis

The Management of Specific Protein Data

Routine Serum Protein Analysis: Trends, Facts and Fallacies

The Use of Discriminant Function Analysis in Selecting Individual Proteins for Routine Clinical Investigation

a2-Antithrombin Levels in Relation to Age and Sex

The Serum Level of ß2-Microglobulin (ß2µ) - A Low Molecular Weight Protein

B3. Pregnancy Levels

Plasma Protein Profiles of Serum and Amniotic Fluid in Normal Pregnancy and Pre-Eclampsia

a1-Fetoprotein in Pregnancy

B4. Profiles Typical of a Disorder

B4.1. Alpha-Antitrypsin System

Purification of a1-Antitrypsin Ultilizing Thiol-Disulphide Interchange

Prevalence of Pi Types Among Newborns of Different Ethnic Backgrounds

The Alpha1-Antitrypsin Null Gene Frequency

Pi MRouen Phenotype in a Pi-Subject

A New Deficiency Allele of Alpha1-Antitrypsin: Pi MMalton

Proteinase Inhibitors in Bronchial Secretions from Patients with Alpha-1-Antitrypsin Deficiency

Alpha-1-Antitrypsin Deficiency and Adult Liver Disease

High Serum Complement Component Levels in Patients with Alpha-1-Antitrypsin Deficiency

B4.2. Immune Disorders

Immunoglobulin Abnormalities in Primary Immunodeficiency Diseases

Immune System Disorders in Man and in Experimental Models accompanied by the Production of Homogeneous Immunoglobulins-Paraproteins

A New Case of IgE Myelomatosis

Imbalances of K/ Immunoglobulin Light Chain Ratios in Normal Individuals and in Immunodeficient Patients

On the Idiotypic Determinants of Human Paraproteins: A Family Study

B4.3. Liver and Renal Diseases

Changes of the Dye-Binding Properties of Serum Proteins in Liver Diseases

Plasma Protein Pattern in Hepatitis…