Protein Engineering for Therapeutics, Part A

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

• Chapters provide an invaluable resource for academics, researchers and students alike
• Iinternational board of authors
• This volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

Contenu

1. Simplified Synthetic Antibody Libraries

2. Generation of Dual Variable Domain Immunoglobulin Molecules for Dual-specific Targeting

3. Discovery of internalizing antibodies to tumor antigens from phage libraries

4. Optimizing properties of anti-receptor antibodies using kinetic computational models and experiments

5. Cell-specific siRNA delivery by peptides and antibodies

6. Conjugation of Anticancer Drugs Through Endogenous Monoclonal Antibody Cysteine Residues

7. Building and characterizing antibody-targeted lipidic nanotherapeutics

8. Cell-Targeting Fusion Constructs Containing Recombinant Gelonin

9. Stapled Peptides for Intracellular Drug Targets

10. Mapping of vascular zip codes by phage display

11. Engineering cyclic peptide toxins

12. Peptide Discovery using Bacterial Display and Flow Cytometry

13. Oral enzyme therapy for celiac sprue

14. Rational Design and Evaluation of Mammalian Ribonuclease Cytotoxins

15. Engineering Reduced-Immunogenicity Enzymes for Amino Acid Depletion Therapy in Cancer

16. Designed Ankyrin Repeat Proteins (DARPins): From research to therapy

17. Target-binding proteins based on the tenth human fibronectin type III domain (10Fn3)

18. Anticalins: small engineered binding proteins based on the lipocalin scaffold

19. T Cell Receptor Engineering

20. Engineering Knottins as Novel Binding Agents

21. Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier

22. Practical Theoretic Guidance for the Design of Tumor Targeting Agents

23. Fusions of elastin-like polypeptides to pharmaceutical proteins

24. Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells