Bachelor Thesis from the year 2020 in the subject Medicine - Pharmacology, grade: A, , course: Biotehcnology, language: English, abstract: Pleurotus ostreatus is primarily an edible mushroom with high nutritional value and biomedical relevance, as it contains numerous bioactive components that generate the development of therapeutic effects. The purpose of this study is to determine the phytopharmacological effects of Pleurotus ostreatus. FT-IR analysis revealed the presence of cycloalkane, nitriles, halogen compounds, aromatic monosubstituted compounds, and carboxyl acid. The presence of 12 bioactive compounds was revealed using gas chromatography-mass spectrometry (GC-MS), and components in the extract were identified and quantified using HPLC. The antimicrobial efficacy of Pleurotus ostreatus aqueous extract against Bacillus megaterium and Bacillus amyloliquifaciens seems promising. The extract's anticancer efficacy against HeLa and MCF7 at 25 µg/ml and exhibits strong cytotoxicity with an IC50 of 6.8 µg/mL and 17.5 µg/mL respectively.