BeadChip Molecular Immunohematology

We have entered the era of molecular immunohematology in which DNA is analyzed to predict red blood cell and platelet phenotypes. Indeed, many laboratories and blood banks across the world have already adopted and implemented molecular techniques. Some fear that serology is now becoming outdated, but immunohematology techniques often supplement the work of serologists, and several leading serology laboratories are the very same ones that were early adopters of molecular immunohematology. This book is a thorough review of molecular immunohematology and is aimed at anyone interested in how it is changing blood bank and transfusion medicine. BeadChip Molecular Immunohematology opens with a history of serology before it provides an overview of gene expression, how genetic variations translate into antigenic differences on the surfaces of cells, and techniques that have been developed to detect genetic variations. It then describes in detail the BeadChip technology developed by BioArray Solutions, the molecular immunohematology system that has been most widely adopted in blood blank laboratories. The remainder of the book focuses on analyses and case studies from around the world to describe how molecular immunohematology is being used in various settings for various purposes. It includes a discussion of the significant advances that have been made to incorporate Rh testing on the BeadChip platform as well as the potential use of that system.

Résumé
This text is a review of molecular immunohematology (MI). It draws from analyses and case studies around the world and details many techniques used in many labs. It is aimed at anyone interested in how MI is changing blood bank and transfusion medicine.

Contenu
An overview of the Classical Serological methods: limitations and benefits of serology and DNA testing.- Introduction to Molecular Typing.- The BeadChip System: A Flexible Array Format for Complex Nucleic Acid and Protein Analysis.- Implementation of HEA at Blood Centers: Prescreening, Rare Donors, Inventory Management.- Implementation of HEA BeadChip System at Medical Centers: Providing Extended Matched Units and Eliminating Complex Workups for Patients.- Human Platelet Antigen Genotyping and Diagnosis of Antiplatelet Alloimmunization.- Blood group genotyping by high-throughput DNA-analysis: Application to the Panel National de Référence du CNRGS.- Implementation and Assessment of High-Throughput Donor Typing at the Milan, Italy Immunohematology Reference Laboratory.- Implementation of the BioArray Erythrocyte Antigen (HEA) genotyping System BeadChip™ HEA: The Red Cross of Spain Experience.- Looking Beyond HEA: Matching SCD Patients for RH Variants Marion E Reid*, Christine Halter Hipsky.- Identification of Altered RHD and RHCE Alleles: A Comparison of Manual and Automated Molecular Methods.- Bayesian Classification Algorithms for Automated Allele Assignment.