New Methods and Sensors for Membrane and Cell Volume Research

New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space - the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest.

• Describes novel FRET-based membrane sensors
• Reviews selected non-optical approaches to membrane structure and dynamics
• Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding

Contenu

On, in, and under membrane M.M. Model and I. Levitan 1. Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell Luca Mantovanelli, Bauke F. Gaastra and Bert Poolman

2. Current methods for studying intracellular liquid-liquid phase separation Amber R Titus and Edgar E Kooijman

3. Investigating molecular crowding during cell division in budding yeast with FRET Sarah Lecinski, Jack W. Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald and Mark C. Leake

4. The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC Yulia Kolobkova, Sumaira Pervaiz and Tobias Stauber

5. Studying cell volume beyond cell volume Michael A. Model

6. Membrane tension Pei-Chuan Chao and Frederick Sachs

7. Methods for assessment of membrane protrusion dynamics Jordan Fauser, Martin Brennan, Denis Tsygankov and Andrei V. Karginov

8. Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids Irena Levitan

9. Fluorescence sensors for imaging membrane lipid domains and cholesterol Francisco J. Barrantes

10. Mass spectrometry-based lipid analysis and imaging Koralege C. Pathmasiri, Thu T.A. Nguyen, Nigina Khamidova and Stephanie M. Cologna

11. Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering Ursula Perez-Salas, Yuri Gerelli, Lionel Porcar and Sumit Garg