Modern methods in protein chemistry

MOD. METH. PROT. CHEM, '82 (TSCHESCHE) E-BOOK

Résumé
Frontmatter -- Preface -- Contents -- Protein Studies as Important Tools in Amplifying Different Characterizations of Biologically Active Macromolecules -- High Resolution Analytical and Preparative Isoelectric Focusing of Proteins: Principles and Strategy -- High Resolution of Complex Protein Solutions by Two- Dimensional Electrophoresis -- Some Recent Developments of the Electroimmunochemical Analysis of Membrane Proteins. Application of Zwittergent, Triton x-114 and Western Blotting Technique -- Affinity Electrophoresis with Special Reference to the Microheterogeneity of Glycoproteins and Identification of Ligand-Binding Proteins -- Principles and Applications of Heterogeneous Enzyme Immunoassays -- Immunological Methods for the Detection of Proteins. Application to Proteins Synthesized in Cell Free Systems in Rat Hepatocytes and by E. Coli Cells Transformed with Recombinant DNA -- Protein Quantification with Zone Immunoelectrophoresis Assay (ZIA) -- High Performance Liquid Chromatography of Peptides and Proteins -- Advanced Automatic Microsequencing of Proteins and Peptides -- The Current Status of Automated Solid-Phase Sequencing -- Microsequence Analysis of Peptides and Proteins -- Aminopeptidase M in the Sequence Analysis of Peptides and Proteins -- Fast Atom Bombardment Mass Spectrometry. A New Technique for Peptide Sequencing. A Review -- Perspectives in the Circular Dichroic Analysis of Protein Main-Chain Conformation -- Spin-Labelled Amino Acids, Peptides and Proteins - Synthesis and Application -- The Ultrastructure of Macromolecular Complexes Studied with Antibodies -- Subject Index -- List of Authors -- Backmatter