Modern Approaches to Animal Cell Technology

Modern Approaches to Animal Cell Technology is a collection of papers presented at the 1987 joint European Society for Animal Cell Technology-OHOLO conference held in Tiberias, Israel. Contributors explore modern approaches to animal cell technology and discuss the construction of the animal cell substrate, the physiology of those cells in a bioreactor type of environment, and the ways in which different products can be made from animal cells in culture and tested.
This book is comprised of 59 chapters divided into nine sections and begins by outlining the history of issues and decisions that were made regarding the acceptability of various cell substrates, along with the use of continuous cell lines in biotechnology. The next chapter explores the feasibility, reproducibility, and the sensitivity of the dot-blot filter hybridization test to detect minute amounts of residual cellular DNA. The reader is then introduced to cells and cell lines such as monoclonal antibodies; cell growth and development; physiology of cells; and the use of bioreactors for culturing animal cells. Downstream unit processes, vaccines, immune system products, and toxicity testing with animal cells are also considered.
This monograph will be a valuable resource for animal-cell technologists, biotechnologists, and microbiologists.

Contenu

1. The Liberation of Animal Cells: Psychology of Changing Attitudes
   
   2. Cellular DNA Biologicals: Significance and Quantitation
      Section 1 New Cells and Cell Lines
2. Human Monoclonal Antibodies - Their Potential, Problems and Prospects
3. Production of Cancer Specific Monoclonal aAtibodies with Human-Human Hybridomas and Their Serum-free, High density, Perfusion Culture
4. Establishment of a Human Hybridoma Secreting Monoclonal Antibodies toward Rabies Virus
5. The Production of Bovine and Ovine Monoclonal Antibodies to Gonadal Steroids: The Use of Interspecific Hybridomas
6. The Immortalization of Differentiated Mammalian Cells
7. Structural Basis of Growth Factor Receptor Mediated Cellular Signals (Abstract)
8. Growth Control of Normal Human B Lymphocytes
9. Anti-proliferative Effects and Phenotypic Alterations Induced by Methylurea Derivatives in B16 Mouse Melanoma
10. Comparative Analysis of Major Biological Aspects of Human Hepatoma in Culture
11. Mini-review: Essential Strategies for the Application of Herpes Simplex Virus as a Vector for the Expression of Foreign Genes
12. The Glutamine Synthetase Gene: A Novel Marker for Gene Amplification in Mammalian Cells (Abstract)
13. Recombinant Animal Cell Lines for Production of Glycoproteins
14. Foreign Gene Expression in Rat Pituitary GH3 Cells
15. Use of Transfected and Amplified Drosophila Heat Shock Promoter Construction for Inducible Production of Toxic Mouse c-myc Proteins in CHO Cells
    Section 2 Medium Development
16. The Use of Glutamine-free Media for the Growth of Three Cell Lines in Microcarrier Culture
17. The Attachment of MDCK Cells to Three Types of Microcarriers in Different Serum-free Media
18. Spreading, Growth and Function of Cells Attached to a Plasma Clot Interphase between Agarose and the Atmosphere
    Section 3 Physiological Investigations
19. Investigations of the Influence of Physical Environment on the Cultivation of Animal Cells
20. Shear Sensitivity of Three Hybridoma Cell Lines in Suspension Culture
21. NMR Metabolic Studies of Human Breast Cancer Cells
22. Optimization of Glass-sphere Immobilized Bed Cultures
23. Examination of a Simple Model for the Diffusion of Oxygen into Dense Masses of Animal Cells
24. Kinetics of Prourokinase Production by Mammalian Cells in Culture
25. Specific Kinetic Patterns of Production of Monoclonal Antibodies in Batch Cultures and Consequences for Fermentation Processes
26. A Model System to Study Gene Expression and Scale up of Microcarrier Cultures of Recombinant DNA Manipulated Cells
    Section 4 Bioreactor Developments
27. Membrane-Stirrer-Reactor for Bubble Free Aeration and Perfusion
28. Immobilization of Hybridomas in Packed-bed Bioreactors
29. Production of Biologicals by Animal Cells Immobilized in Polyurethane Foam Matrix
30. Comparison of Oxygenation Methods in a 39L Stirred Bioreactor
31. Development of Large-scale Mammalian Cell Perfusion Culture Systems
32. Microcarrier DORMACELL does not Detectably Alter Viral or Plasmid DNA upon Direct Exposure
33. Macroporous Microcarriers
34. Growth of Animal Cells on Microbeads III: Semi Continuous in Situ Estimation of Numbers
35. Evaluation of the Continuous Perfusion Culture System for the Production of Monoclonal Antibodies
36. Recent Advances in the Control of Monitoring of Bioreactors
37. Automated Process Control for Culturing Animal Cells
    Section 5 Downstream Unit Processes
38. Design Criteria for the Purification of Biopolymers for the Pharmaceutical Industry
39. Use of a Dynamic Filtration Method for Separation of Animal Cells
40. Continuous Culture of Murine Hybridomas with Integrated Recovery of Monoclonal Antibodies
    Section 6 Vaccine Products
41. Improved Production of Marek's Disease Vaccine with the Multi-tube Technology
42. Procedure for the Adoption of the New Mumps Vaccine Strain 'Rubini' to Human Diploid Cells
43. Immuno Detection of Flavi Viruses by Monoclonal Antibodies Directed against West Nile Virus Specific and Flavivirus Group Epitopes
44. A Simple ELISA for the Estimation of Antibodies to Equine Herpes Virus (EHV-1) and Its Application to the Quality Control of Vaccines
45. The Production and Application of an Oil Adjuvant Vaccine against Foot-and-Mouth Disease in Cattle
    Section 7 Immune System Products
46. Application of Large Scale Perfusion Systems for Pharmaceutical Production from Mammalian Cells
47. The Organotype Culture Model of Human Cancer Cells: The Effect of Human Recombinant Interferons
48. Immune Interferon Enhances the Expression of MHC Antigens in Human Placenta
49. Gamma Interferon and Anti-thy-l Antibody Induce Specific Killing Activity in Memory-like Cytolytic Hybridomas
50. Development of a Device and a Double Antibody Microradioimmune Assay for Fast Screening and Sensitive Quantitation of Monoclonal Antibodies
51. Development of Monoclonal Antibodies Directed against Carcinoembryonic Antigen (CEA) and Their Application for CEA Detection
52. Carcinoembryonic Antigen (CEA) Production in Low Protein Growth Medium
53. Production and Purification of a Bladder Cancer Associated Membrane Protein
    Section 8 Toxicity Testing with Animal Cells
54. The Use of Animal Cells for Evaluation of Toxicity, Carcinogenesis and Mutagenesis
55. Development of a Model for the Study of Toxicity and Carcinogenicity In Vitro 000
    Section 9 A Glimpse of the Future
56. The Transformation of Science Fiction into Technical Reality by Using Animal Cells in Culture
    Postscript
57. Terms and Symbols for Use in Animal Cell Culture
    Late Paper
58. Hybridoma Culture for the Continuous Production of Monoclonal Antibodies; a Fractionalized Factorial Design Experiment for Process Optimization I
    List of Participants
    List of Exhibitors