Microsomes and Drug Oxidations

Microsomes and Drug Oxidations documents the proceedings of symposium on microsomes and drug oxidations held at Bethesda, Maryland, 16-17 February 1968. The theme of the symposium centered on the morphology of the liver endoplasmic reticulum, the localization of enzyme systems, the mechanisms for regulating enzyme activity, and the mechanisms of enzyme action. The results of this symposium presented in this book illustrate the complexity of the enzyme systems under investigation and the multiplicity of the variables altering their function. It is hoped the information presented will serve as a stimulus to other investigators in the elucidation of these problems.
The book contains 27 papers organized into four parts. The papers in Part I examine the morphological and biochemical characteristics of microsomes. Part II presents studies on electron transfer components. Part III examines alterations of microsomal enzymes while Part IV focuses on drugs and protein synthesis.

Contenu

Participants

Preface

I. Morphological And Biochemical Characteristics Of Microsomes

Microsomes, Endoplasmic Reticulum, and Interactions of Cytoplasmic Membranes

Subfractionation of Rat Liver Microsomes

Remarks

A Possible Role of the Endoplasmic Reticulum in the Regulation of Genetic Expression: The MEMBRON Concept

The Metabolism of Drugs by Subfractions of Hepatic Microsomes: The Case for Microsomal Heterogeneity

II. Electron Transfer Components

Organization of the Microsomal Electron Transport System

Discussion

Characterization of Two Hemoproteins of Liver Microsomes

Discussion

Cytochrome P-450 Reduction in Liver Microsomes and Its Relationship to Drug Metabolism

Discussion

Fatty Acid co-Oxidation in a Soluble Microsomal Enzyme System Containing P-450

Discussion

Enzymatic w-Oxidation: Stoichiometry of the Reaction Catalyzed by the Inducible Enzyme System of Pseudomonas Oleovorans

The Properties of a Purified Hepatic Microsomal Mixed Function Amine Oxidase

Discussion

A Comparison of NADPH Dependent Reductive Cleavage and N-Demethylation of Aminoazobenzene, Monomethylaminoazobenzene and Dimethylaminoazobenzene by Cell-Free Preparations from Rat Liver

Oxygen Reactions in Model Systems

Discussion

Significance of the NIH Shift with Respect to Liver Microsome Hydroxylations

Discussion

The Microsomal Hydroxylation of Ethylbenzene: Stereochemical, Induction, and Isotopic Studies

III. Alterations of Microsomal Enzymes

Overall Biochemical Effects of Drug Induction on Liver Microsomes

Discussion

Specificity in the Regulation of the 6ß, 7a, and 16a-Hydroxylation of Testosterone by Rat Liver Microsomes

Discussion

Formation of a New P-450 Hemoprotein after Treatment of Rats with Polycyclic Hydrocarbons

Discussion

Spectrophotometric Studies of Cytochrome P-450 of Liver Microsomes after Induction with Phenobarbital and 3-Methylcholanthrene

Discussion

The Effects of 3-Methylcholanthrene and Phenobarbital on Liver Microsomal Hemoproteins and on the Hydroxylation of Benzpyrene

Discussion

Spectral Investigations on Cytochrome P-450

Discussion

IV. Drugs and Protein Synthesis

Drugs and Microsomal Enzyme Formation In Vivo and in Mammalian Cell Culture

Discussion

Activation of Chromatin by 3-Methylcholanthrene

Discussion

Induction, Stabilization, and Turnover of Endoplasmic Reticulum Proteins

Discussion

Effect of Phenobarbital on the Turnover of Microsomal Enzymes

Discussion

The Role of Heme in the Synthesis and Repression of Microsomal Protein

Discussion

Insect Microsomes and Insecticide Chemical Oxidations

Discussion

Stimulatory Effect of Acetone on the Activity of Microsomal Aniline Para-Hydroxylase

Subject Index