Methods of Enzymatic analysis V4

Methods of Enzymatic Analysis, Volume 4 reviews developments in the use of enzymes as tools in analytical biochemistry, including advances in assay techniques. It discusses the principles and methods for the elucidation of structures of enzymes, such as peptides, proteins, amino acids, fatty acid metabolites, lipids, steroids, nucleic acids, purines, pyrimidines, nucleosides, and coenzymes. It also considers the isolation and characterization of active centers in enzymes.
This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on proteins, peptides, and amino acids including amines and amides. Part II is concerned with fatty acid metabolites, lipids, and steroids ranging from polyunsaturated fatty acids and lecithin to choline, acetylcholine, triglycerides, glycerol, acetoacetate, triacetate, fumarylacetoacetate, 20-ketosteroids, prostaglandins, bile acids, and cholesterol. Part III discusses nucleic acids, purines, pyrimidines, nucleosides, coenzymes, and related compounds, whereas Part IV looks at other substrates and effectors such as inorganic phosphate. The book concludes with a chapter on metabolites and their concentrations in animal tissues.
Biochemists as well as students and researchers working in the field of analytical biochemistry will find this book highly informative.

Contenu

Preface

From the Preface to the 1st Edition

Volume I

Contents

Contributors

Abbreviations

Section A: General Introduction

I. The Importance of Enzymatic Analysis

1. in Biochemistry

2. in Medicine

3. in Foodstuff Chemistry

4. in Botany and Agricultural Chemistry

5. in Microbiology

   II. Principles of Enzymatic Analysis

6. Introduction

7. Reaction Kinetics

8. Determination of Concentration of Metabolites (End-point Methods)

9. Determination of Enzyme Activities

10. Determination of Concentrations by Kinetic Methods

11. Kinetics of "Enzymatic Cycling"

12. Visualization of NAD(P)-dependent Reactions

13. Determination of Michaelis Constants and Inhibitor Constants

    III. Experimental Techniques

14. Handling of Biochemical Reagents and Samples

15. Methods of Measurement and Instruments Used

16. Evaluation of Experimental Results

    IV. Statistical Analysis, Control and Assessment of Experimental Results

17. Introduction

18. Statistical Concepts and Methods

19. Quality Control

20. Assessment of Results

    V. Cell and Tissue Disintegration

21. General

22. Methods for Animal Tissue and Micro-organisms

23. Methods for Plant Tissues

Section B: Biochemical Reagents

I. Reagents for Enzymatic Analysis

1. Nomenclature and Standardization

2. Quality Requirements

3. Suppliers of Biochemical Reagents

   II. Enzymes as Biochemical Reagents

   III. Coenzymes, Metabolites and other Biochemical Reagents

   IV. Complete Reagent Kits

4. For Determination of Metabolites

5. For Determination of Enzyme Activities

6. Main Manufacturers and Suppliers

Index

Volume II

Contents

Contributors

Abbreviations

Section C: Methods for Determination of Enzyme Activity

I. Oxidoreductases

1. Sorbitol Dehydrogenase

2. Lactate Dehydrogenases

3. Malate Dehydrogenase

4. Isocitrate Dehydrogenase

5. 6-Phosphogluconate Dehydrogenase

6. Glucose-6-phosphate Dehydrogenase

7. Xanthine Oxidase

8. Glutamate Dehydrogenase

9. Diamine Oxidase

10. Dihydrofolate Reductase (Tetrahydrofolate Dehydrogenase)

11. Catalase

12. Peroxidases

    II. Transferases

13. Ornithine Carbamoyltransferase

14. Transamidinase

15. Transketolase

16. Transaldolase

17. -Glutamyltranspeptidase

18. UDP-Glucuronyltransferase

19. Aminotransferases and Related Enzymes

20. Phosphotransferases

    III. Hydrolases

21. Esterases

22. Glycoside Hydrolases

23. Peptidases and Proteinases

24. Other C-N splitting Hydrolases

    IV. Lyases, Isomerases, Ligases

25. Fructose-1,6-diphosphate Aldolase

26. 1-Phosphofructoaldolase

27. Glucosephosphate Isomerase

28. Tetrahydrofolate Formylase

Index

Volume III

Contents

Contributors

Abbreviations

Section D. Methods for Determination of Metabolites

I. Carbohydrate Metabolites

1. Poly-, Oligo- and Disaccharides

2. Monosaccharides and Derivatives

3. Three Carbon compounds

4. Two and One Carbon Compounds

   II. Citric Acid Cycle Metabolites

5. Citrate

6. Isocitrate

7. 2-Oxoglutarate

8. L-(-)-Malate

9. L-Malate and Fumarate

10. Oxaloacetate

11. Succinate

12. Maleate

Index

Volume IV

Contents

Contributors

Abbreviations

III. Proteins, Peptides and Amino Acids

1. Proteins and Peptides

2. Amino Acids, Amines and Amides

   IV. Fatty Acid Metabolites, Lipids and Steroids

3. Polyunsaturated Fatty Acids

4. Lecithin

5. Acetylcholine and Choline

6. Triglycerides and Glycerol Determination after Alkaline Hydrolysis

7. Triglycerides, Determination after Enzymatic Hydrolysis

8. D-(-)-3-Hydroxybutyrate

9. Acetoacetate

10. Triacetate and Fumarylacetoacetate

11. Hydrolysis of Steroid Conjugates

12. 20-Ketosteroids

13. Steroid Alcohols in Urine

14. Prostaglandins

15. Bile Acids

16. Cholesterol and Esterified Cholesterol

    V. Nucleic Acids, Purines, Pyrimidines, Nucleosides, Coenzymes and Related Compounds

17. Nucleic Acids and Polyribonucleotides

18. Purines, Pyrimidines and Nucleosides

19. Nucleotides, Coenzymes and Related Compounds

20. Nucleoside Diphosphate Sugars and Derivatives

    VI. Other Substrates and Effectors

21. Inorganic Phosphate

22. Inorganic Pyrophosphate

23. Inorganic Peroxides

24. Organophosphorus and Carbamate Insecticides

25. Nitrate

    Concentrations of Metabolites in Animal Tissues

26. Glycogen, Glucose and Glycolytic Intermediates

27. Tricarboxylic Acid Cycle Intermediates

28. Amino Acids and Ammonia

29. Pentose Phosphate Pathway Intermediates

30. Coenzyme A and Intermediates of Lipid Metabolism

31. Adenosine and other Nucleotides

32. Nicotinamide-Adenine Dinucleotides

33. Creatine Phosphate, Creatine, Inorganic Pyrophosphate and Phosphate

Index