Methods of Enzymatic Analysis V2

Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol.
This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases. Part III discusses hydrolases including esterases, glycoside hydrolases, peptidases, and proteinases, whereas Part IV looks at lyases, isomerases, and ligases, such as fructose-1, 6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase.
This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.

Contenu

Preface
From the Preface to the 1st Edition
Volume I
Contents
Contributors
Abbreviations
Section A: General Introduction
I. The Importance of Enzymatic Analysis

1. in Biochemistry
2. in Medicine
3. in Foodstuff Chemistry
4. in Botany and Agricultural Chemistry
5. in Microbiology
   II. Principles of Enzymatic Analysis
6. Introduction
7. Reaction Kinetics
8. Determination of Concentration of Metabolites (End-point Methods)
9. Determination of Enzyme Activities
10. Determination of Concentrations by Kinetic Methods
11. Kinetics of "Enzymatic Cycling"
12. Visualization of NAD(P)-dependent Reactions
13. Determination of Michaelis Constants and Inhibitor Constants
    III. Experimental Techniques
14. Handling of Biochemical Reagents and Samples
15. Methods of Measurement and Instruments Used
16. Evaluation of Experimental Results
    IV. Statistical Analysis, Control and Assessment of Experimental Results
17. Introduction
18. Statistical Concepts and Methods
19. Quality Control
20. Assessment of Results
    V. Cell and Tissue Disintegration
21. General
22. Methods for Animal Tissue and Micro-organisms
23. Methods for Plant Tissues
    Section ?: Biochemical Reagents
    I. Reagents for Enzymatic Analysis
24. Nomenclature and Standardization
25. Quality Requirements
26. Suppliers of Biochemical Reagents
    II. Enzymes as Biochemical Reagents
    III. Coenzymes, Metabolites and other Biochemical Reagents
    IV. Complete Reagent Kits
27. For Determination of Metabolites
28. For Determination of Enzyme Activities
29. Main Manufacturers and Suppliers
    Index
    Volume II
    Contents
    Contributors
    Abbreviations
    Section C: Methods for Determination of Enzyme Activity
    I. Oxidoreductases
30. Sorbitol Dehydrogenase
31. Lactate Dehydrogenases
32. Malate Dehydrogenase
33. Isocitrate Dehydrogenase
34. 6-Phosphogluconate Dehydrogenase
35. Glucose-6-phosphate Dehydrogenase
36. Xanthine Oxidase
37. Glutamate Dehydrogenase
38. Diamine Oxidase
39. Dihydrofolate Reductase (Tetrahydrofolate Dehydrogenase)
40. Catalase
41. Peroxidases
    II. Transferases
42. Ornithine Carbamoyltransferase
43. Transamidinase
44. Transketolase
45. Transaldolase
46. ?-Glutamyltranspeptidase
47. UDP-Glucuronyltransferase
48. Aminotransferases and Related Enzymes
49. Phosphotransferases
    III. Hydrolases
50. Esterases
51. Glycoside Hydrolases
52. Peptidases and Proteinases
53. Other C-N splitting Hydrolases
    IV. Lyases, Isomerases, Ligases
54. Fructose-1,6-diphosphate Aldolase
55. 1-Phosphofructoaldolase
56. Glucosephosphate Isomerase
57. Tetrahydrofolate Formylase
    Index
    Volume III
    Contents
    Contributors
    Abbreviations
    Section D. Methods for Determination of Metabolites
    I. Carbohydrate Metabolites
58. Poly-, Oligo- and Disaccharides
59. Monosaccharides and Derivatives
60. Three Carbon compounds
61. Two and One Carbon Compounds
    II. Citric Acid Cycle Metabolites
62. Citrate
63. Isocitrate
64. 2-Oxoglutarate
65. L-(-)-Malate
66. L-Malate and Fumarate
67. Oxaloacetate
68. Succinate
69. Maleate
    Index
    Volume IV
    Contents
    Contributors
    Abbreviations
    III. Proteins, Peptides and Amino Acids
70. Proteins and Peptides
71. Amino Acids, Amines and Amides
    IV. Fatty Acid Metabolites, Lipids and Steroids
72. Polyunsaturated Fatty Acids
73. Lecithin
74. Acetylcholine and Choline
75. Triglycerides and Glycerol Determination after Alkaline Hydrolysis
76. Triglycerides, Determination after Enzymatic Hydrolysis
77. D-(-)-3-Hydroxybutyrate
78. Acetoacetate
79. Triacetate and Fumarylacetoacetate
80. Hydrolysis of Steroid Conjugates
81. 20-Ketosteroids
82. Steroid Alcohols in Urine
83. Prostaglandins
84. Bile Acids
85. Cholesterol and Esterified Cholesterol
    V. Nucleic Acids, Purines, Pyrimidines, Nucleosides, Coenzymes and Related Compounds
86. Nucleic Acids and Polyribonucleotides
87. Purines, Pyrimidines and Nucleosides
88. Nucleotides, Coenzymes and Related Compounds
89. Nucleoside Diphosphate Sugars and Derivatives
    VI. Other Substrates and Effectors
90. Inorganic Phosphate
91. Inorganic Pyrophosphate
92. Inorganic Peroxides
93. Organophosphorus and Carbamate Insecticides
94. Nitrate
    Concentrations of Metabolites in Animal Tissues
95. Glycogen, Glucose and Glycolytic Intermediates
96. Tricarboxylic Acid Cycle Intermediates
97. Amino Acids and Ammonia
98. Pentose Phosphate Pathway Intermediates
99. Coenzyme A and Intermediates of Lipid Metabolism
100. Adenosine and other Nucleotides
101. Nicotinamide-Adenine Dinucleotides
102. Creatine Phosphate, Creatine, Inorganic Pyrophosphate and Phosphate
     Index