Membrane Proteins - Engineering, Purification and Crystallization

Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.

• Volume of Methods In Enzymology
• Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Contenu

Section I. Membrane Protein Engineering, Solubilization and Purification

1. Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes
   Simon Trowitzsch & Robert Tampé
2. A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs
   Elizabeth Mathew and Mark E Dumont
3. Membrane preparation and solubilization
   Ankita Roy
4. Amphipathic agents for membrane protein study
   Aiman Sadaf, Kyung Ho Cho, Bernadette Byrne, Pil Seok Chae
5. Quantification of detergent using colorimetric methods in membrane protein crystallography
   Chelsy Prince , Zongchao Jia
6. Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to Explore Lipid-Receptor Interaction
   Amitabha Chattopadhyay, Bhagyashree D. Rao and Md. Jafurulla
7. Overexpression, Isolation, Purification and Crystallization of NhaA
   Etana Padan and Manish Dwivedi
8. Purification, refolding and crystallization of the outer membrane protein OmpG from Escherichia coli
   Stefan Köster, Katharina van Pee, Özkan Yildiz
9. Biophysical Approaches to the Study of LeuT, a Prokaryotic Homolog of Neurotransmitter Sodium Symporters
   Satinder K. Singh and Aritra Pal
   Section II. Generation and Use of Antibody Fragments Against Membrane Proteins
10. Generation of recombinant antibody fragments for membrane protein crystallization
    Syed H. Mir , Claudia Escher, Wei-Chun Kao, Dominic Birth, Christophe Wirth, Carola Hunte
11. Phage Display Selections for Affinity Reagents to Membrane Proteins in Nanodiscs
    Pawel K. Dominik, and Anthony A. Kossiakoff
12. Antibody fragments for crystallization, trapping of active conformations and stabilization of G-protein coupled receptors and their signaling complexes
    Arun K. Shukla, Charu Gupta, Ashish Srivastava and Deepika Jaiman
    Section III. Biophysical Studies of Membrane Proteins
13. Conformational Analysis of G Protein-Coupled Receptor Signaling by Hydrogen/Deuterium Exchange Mass Spectrometry
    Sheng Li, Su Youn Lee and Ka Young Chung
14. EPR Studies of Gating Mechanisms in Ion Channels
    Sudha Chakrapani
15. Magic-Angle-Spinning Solid-State NMR of Membrane Proteins
    Lindsay A Baker, Gert E Folkers, Tessa Sinnige, Klaartje Houben, Mohammed Kaplan, Elwin AJ van der Cruijsen, Marc Baldus
16. Solution NMR Structure Determination of Polytopic a-helical membrane proteins: A guide to spin label paramagnetic relaxation enhancement restraints
    Linda Columbus and Brett Kroncke
    Section IV. Crystallization of Membrane Proteins
17. Inducing Two-Dimensional Crystallization of Membrane Proteins by Dialysis for Electron Crystallography
    Yusuf M. Uddin and Ingeborg Schmidt-Krey
18. Crystallization of Membrane Proteins by Vapor Diffusion
    Jared A. Delmar, Jani Reddy Bolla, Chih-Chia Su, and Edward W. Yu
19. An empirical approach to bicelle crystallization
    Sandra Poulos, Jacob L. W. Morgan, Jochen Zimmer, Salem Faham
20. Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP): A pre-crystallization assay for membrane proteins
    Gustavo Fenalti, Enrique E. Abola, Chong Wang, Beili Wu, Vadim Cherezov
21. Crystallization of Proteins from Crude Bovine Rod Outer Segments
    Bo Y Baker, Sahil Gulati, Wuxian Shi, Benlian Wang, Phoebe L. Stewart, and Krzysztof Palczewski
22. Crystallization of Photosystem II for Time Resolved Structural Studies Using an X-ray Free Electron Laser
    Jesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme
    Section V. Computational Approaches to Understand Membrane Proteins
23. Major Intrinsic Protein Superfamily: Channels with Unique Structural Features and Diverse Selectivity Filters
    Ravi Kumar Verma, Anjali Bansal Gupta and Ramasubbu Sankararamakrishnan
24. Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: the 'Mix-and-Match' Method
    M. Gregor Madej
25. Elucidating Ligand-Modulated Conformational Landscape of GPCRs Using Cloud-computing Approaches
    Diwakar Shukla, Morgan Lawrenz and Vijay S. Pande