Mechanisms of Cytotoxicity by NK Cells

Mechanisms of Cytotoxicity by NK Cells is the resulting book of a workshop focused on the studies that enhance understanding of the basic mechanisms involved in the function and regulation of NK cells. The book presents the status of knowledge of the mechanisms responsible for the cytotoxic effects of NK cells and the regulation of their activity.
The text is further divided into five major sections according to the sequence of the workshop sessions. A separate chapter from the sections has been included at the beginning of the book. The introductory chapter clarifies some points in the subject area of NK cells, particularly its definition. The heterogeneity of NK cells is also discussed in this chapter. Then, the book presents the major topics, including the purification and characterization of NK cells and NK target structures and recognition sites. The mechanisms of postrecognition interactions between NK cells and targets; regulation of NK activity and NK cells; and cultured cells with NK-like activity are also discussed.
This book will serve as a helpful guide to students and professionals (scientists, researchers, and professors) in the fields of immunology, biology, microbiology, biochemistry, among others.

Contenu

Contributors
Preface
I Purification and Characterization of NK Cells
Purification and Characterization of NK Cells
Surface Phenotype and Functions of Human Natural Killer Cells
Characteristics of Mouse NK Cells and Their Precursors
Enrichment Methodology for Murine NK Activity
II Characterization of NK Target Structures and Recognition Sites on NK Cells
Molecular Characterization of NK Target Antigens and Target Recognition Sites on NK Cells
Analysis of NK Target Structures. K562 Membrane Components Associated with Susceptibility to NK Attack
Role of Target Histocompatibility Antigens in Regulation of Natural Killer Activity: A Reevaluation and a Hypothesis
Role of NK Cells in Hybrid Resistance to Bone Marrow Grafts
Evidence That Natural Killer Cells Are Pre-Thymic T Cells with Receptors for Self and Non-Self H Antigens
Receptors for "Self" Recognition Shared by Human T Lymphocytes and NK Cells
Analysis of NK Activity against Virus-Infected Target Cells
III Mechanisms of Postrecognition Interactions between NK Cells and Targets
Biochemistry of NK Cytotoxicity
NK-Target Cell Interactions in Binding, Triggering, Programming, and Lethal Hit Stages of NK Cytotoxicity
NK Mediated Cell Lysis: A Directed Secretory Event of Cytolytic Tubular Structures
Involvement of the Cytoskeleton and an Associated 95 KD Protein in Recognition and Lysis by NK Cells
Ultrastructural Analysis of Human Natural Killer Cell-Target Cell Interactions Leading to Target Cell Lysis
Role of Proteases in NK Activity
Enzymes Involved in Natural Killer Cell Activity: Evidence for Phospholipases, Lipoxygensases, and Serine Dependent Proteinases
The Role of Phospholipid Methylation and Chromosomally Regulated Target Structures in Pre- and Post-Recognition Events in the NK Cytolytic Pathway
Monosaccharide Concentration Dependent Inhibition of Human Natural Cytotoxicity
The Potential Role of Arachidonic Acid Metabolism in Human Natural Killer Cell Activity
The Role of the Lipoxygenase in Regulation of NK Activity
No Confirmed Role for Reactive Oxygen Intermediates in Natural Killer (NK) Cell-Mediated Cytolysis
Oxidative Burst Does Not Mediate Human Monocyte Tumoricidal Activity
The Role of Cytoplasmic Granules in NK Cell Cytotoxicity
Functional and Biochemical Characterization of Natural Killer Cytotoxic Factors and Their Role in the NK Lytic Mechanism
Characteristics of Human Natural Killer Cytotoxic Factor (NKCF) Lysis and Possible Relationship to Lysis by NK Cells
Role of Soluble Lytic Factors in NK Activity
Cytotoxic Factors from Marmoset Monkey NK-Like Cell Lines
IV Regulation of NK Activity and Regulation by NK Cells
Regulation of NK Cells
Kinetic Models for Natural Cytotoxicity and Their Use for Studying Activated NK Cells
Augmentation of Natural Killer Cell Activity by Recombinant Interleukin-2 and Recombinant Interferons
Lymphokines Involved in the Generation and Regulation of NK and NC Cells
Regulation of Mouse NK Activity
Alterations in Numbers of LGLs as Important Component of Regulation of NK Activity
Effects of Phorbol Esters on NK Activity
Characterization of Soluble Suppressor Factors Produced by Unstimulated Normal Human Lymphocytes
Role of Transferrin and Transferrin-Related Proteins in Regulation of NK Activity in Vitro
Effect of UVR and Membrane Fluidity on NK Cytotoxic Activity
Immunoregulatory Effects of NK Cells
Regulation of the B Cell Response by NK Cells
Defective NK Cell Mechanism in Patients with Leukemia
Characterization of NK Cells in Patients with Chronic Myelogenous Leukemia
Natural Killer Cell Activity in Genetic Diseases
V Cultured Cells with NK-Like Activity: Models for Analysis of NK Activity
NK Clones: Isolation and Characterizations
K562 Tumor Cells Preferentially Activate Leu 11 + Human Large Granular Lymphocytes in a Mixed Lymphocyte Response Culture
Discrimination between Anomolous Killer Cells and NK-Like Cells
Differentiation of Large Granular Lymphocytes during Induction of Activated Killer Cells in Vitro
Patterns of Proliferation in Human NK Cells: Practical and Theoretical Implications
Culture of NK Cells: Specificity, Phenotypes, Functions, and Comparison with Activated T Cells
Analysis and Functional Characterization of T3-, NK Cell Derived and T3+, CTL Precursor Cell Derived Clones
NK Cells and T Cell Differentiation
Recognitive Heterogeneity of NK Cells
Index