Laboratory Hematology Practice

Expertly edited and endorsed by the International Society for Laboratory Hematology, this is the newest international textbook on all aspects of laboratory hematology. Covering both traditional and cutting-edge hematology laboratory technology this book emphasizes international recommendations for testing practices. Illustrative case studies on how technology can be used in patient diagnosis are included. Laboratory Hematology Practice is an invaluable resource for all those working in the field.

Auteur

Dr Kandice Kottke-Marchant, MD PhD, Section Head, Hemostasis
and Thrombosis, Department of Clinical Pathology, The Cleveland
Clinic Foundation, Cleveland, Ohio, USA and President,
International Society of Laboratory Hematology. She is also a
Professor of Pathology at the Cleveland Clinic Lerner College of
Medicine and an Adjunct Professor of Biomedical Engineering at Case
Western Reserve University. She has published over 100
peer-reviewed publications in the field of haemostasis, is an
international expert on platelet testing and aspirin resistance,
and has served on review panels from the National Institutes of
Health (NIH), College of American Pathologists (CAP), ISO/TC212 and
North American Specialized Coagulation Laboratory Association
(NASCOLA). She has been a driving force to increase the
international visibility and impact of the ISLH. She has been
responsible for running the ISLH annual Symposia since 2002. Due to
her involvement in haemostasis and the ISLH, Dr. Kottke-Marchant
will be uniquely positioned to serve as one of the co-Editors of
the Textbook of Laboratory Hematology.

Elkin Simson, MD, PCAP, FACSP is the Medical Director of
the Center for Clinical Laboratories and Hematology Laboratory at
the Mount Sinai Hospital. He is also Associate Professor and Vice
Chairman of the Department of Pathology at the Mount Sinai School
of Medicine, New York, NY, USA. Dr. Simson was formerly the
Vice-President for Medical and Scientific Affairs at Technicon
Instruments Corporation, where he participated in the development
of the first automated haematology analyzers to combine the CBC and
WBC differential into a single instrument. Dr Simson is the Past
President of the ISLH and is Vice-President of the International
Council for Standardization in Hematology (ICSH). He has been
Chairman of the Hematology Area Committee of NCCLS (now CLSI), a
member of Council of the National Reference System for the Clinical
Laboratory (NRSCL) and the Hematology and Quality Improvement
Subcommittees of the NCCCLS. He was a member of the Hematology and
Pathology Advisory Panel of the Food and Drug Administration (FDA).
Due to his long-time involvement in haematology automation and
standards organizations, Dr. Simson is an idea co-editor.

The proposed section editors are chosen from international
experts in their respective fields; most have also been long-time
ISLH members who have been faculty at one or more ISLH Symposia and
who have been repeatedly recognized as outstanding speakers and
contributors by the participants. Dr Bruce Davis is one of
the co-founders of the ISLH and has a long history of involvement
in developing automated cellular and flow cytometric technologies.
Professor Alberto Orfao from Spain is one of the world's
foremost experts on flow cytometry. Professor Samuel Machin is
currently President of the British Society of Haematology and is an
international authority on haemostasis testing. Stefanie McFadden
is a medical technologist who is sought after as a consultant for
laboratory management and informatics issues.

Contenu
Contributors, viii Preface, xiii

Abbreviations, xv

I Cellular Analysis, 1

1 Historical Perspective on Cellular Analysis, 3
Elkin Simson

2 Cellular Morphologic Analysis of Peripheral Blood, 10
Powers Peterson, Sheila McNeill, and Gene Gulati

3 Automated Cell Analysis: Principles, 26
Bruce H. Davis and Patrick W. Barnes

4 Differential Leukocyte Analysis, 33
Marie-Christine Béné and Francis Lacombe

5 Automated Platelet Analysis, 48
Carol Briggs and Samuel J. Machin

6 Method Standardization in Cellular Analysis, 59
Ian Giles & Richard Kendall

7 Spurious counts and spurious results on hematology analyzers: platelets, 66
Marc Zandecki, Franck Genevieve, Jérémie Gérard, and Alban Godon

8 Spurious Counts and Spurious Results on Hematology Analyzers: White Blood Cells, Red Blood Cells, Hemoglobin, Red Cell Indices, and Reticulocytes, 79
Marc Zandecki, Franck Genevieve, Jérémie Gérard, and Alban Godon

9 Evaluation of Hematology Analyzers, 96
Carol Briggs

II Flow Cytometry in Hematologic Diagnosis, 103

10 Flow Cytometric Specimen Collection, Processing, and Reporting, 105
Maryalice Stetler-Stevenson, Bruce Greig, Constance Yuan

11 Validation and Quality Control in Clinical Flow Cytometry, 115
Norman B. Purvis and Teri Oldaker

12 A New Paradigm for Cytometric Analysis, 131
C. Bruce Bagwell

III Molecular Diagnostics, 143

13 Sample Collection, Processing, and Storage for Molecular Genetic Testing, 145
Bobby L. Boyanton Jr. and Domnita Crisan

14 Development of Amplification-Based Molecular Genetic Testing in Hematology, 155
Raymond R. Tubbs

15 Single Nucleotide Polymorphisms in Molecular Diagnostics, 168
Gurunathan Murugesan, Seongsoo Jang, and Jin-Yeong Han

16 Molecular Cytogenetic (FISH) Analysis of Hematolymphoid Disorders, 181
Mark A. Micale and Anwar N. Mohamed

17 Immunoglobulin and T-Cell Receptor Gene Rearrangement Analysis for Diagnosis of Hematologic Malignancies, 199
Jacques J. M. van Dongen, Anton W. Langerak, and Tomasz Szczepan ski

18 Minimal Residual Disease Detection in Acute Myeloid Leukemia by RQ-PCR and Multiparameter Flow Cytometry, 220
Wolfgang Kern and Susanne Schnittger

19 Engraftment Analysis using Short Tandem Repeats following Allogeneic Hematopoietic Cell Transplantation, 234
Ilka Warshawsky and Hyun-Sook Chi

20 Molecular Profiling Methods in the Diagnosis of Hematologic Disorders, 244
Annette S. Kim, Stephen R. Master, and Cherie H. Dunphy

21 The Role of Classical Cytogenetics in Hematologic Diagnosis, 267
Kathleen Richkind

IV Bone Marrow Analysis, 279

22 Bone Marrow Processing and Normal Morphology, 281
Karl S. Theil

23 Cytochemical, Immunocytochemical, Histochemical, and Immunohistochemical Staining of Bone Marrow and Peripheral Blood, 300
Alexandra Rice and Barbara J. Bain

24 The Myeloproliferative Neoplasms, 315
Sindhu Cherian and Brent L. Wood

25 Myelodysplastic Disorders, 331
Maryalice Stetler-Stevenson, Joseph A. DiGiuseppe, and Diane C. Arthur

26 Acute Leukemias, 345
Joseph A. DiGiuseppe and Laila Mnayer

27 Lymphoproliferative Disorders, 364
Robert W. Allan, Samer Z. Al-Quran, Ying Li, and Raul C. Braylan

28 Plasma Cell Disorders, 381
Bill G. Richendollar and James R. Cook 29 Diagnosis of Adult Mastocytosis: Role for Bone Marrow Analysis, 388
*Luis Escribano, Andrés Garcia-Montero, Laura Sanchez-Muñoz, Cristina Teodosio, Ivan Alvarez-Twose, Maria Jara-Acevedo, José Mario Morgado, Julia Almeid...