Methods of Protein Microcharacterization

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Contenu

Microisolation Techniques.- Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography.- Reverse-Phase HPLC Isolation and Microsequence Analysis.- Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis.- Microamino Acid Analysis.- Amino Acid Analysis With Postcolumn Fluorescent Derivatization.- Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC.- Amino Terminal Analysis.- Manual Edman Sequencing System.- Comparison of the Spinning Cup and Gas-Phase Instruments.- Gas-Phase Protein/Peptide Sequencer.- Design of a Multipurpose Sequencer.- Solid-Phase Methods in Protein Microsequence Analysis.- Analysis of Phenylthiohydantoin Amino Acids by HPLC.- Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation.- Carboxyl-Terminal Analysis.- Microsequence Analysis by Enzymatic Methods.- COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods.- Mass Spectrometric Analysis of Polypeptides.- Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry.- Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.