Invertebrate Tissue Culture

Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture comprises the proceedings of the IV International Conference on Invertebrate Tissue Culture, held on June 5-8, 1975 at Mont Gabriel, Quebec, Canada. The conference focuses on invertebrate organ, tissue, and cell culture, as well as cell culture limitations, pitfalls, and applications in medicine, biology, agriculture, neurophysiology, and studies of morphogenesis, differentiation, viruses, symbionts, and parasites.
This reference material specifically provides information on sophisticated laboratory methods and on numerous utilizations of invertebrate cell culture techniques in medicine and biology. This book also elucidates the nutritional requirements and the establishment of cell lines. The study of viruses and protozoa of agricultural and forest importance is also shown.
This book will be useful and stimulating to the readers and will provide in a single volume the results obtained in the diverse areas of research pursued by the leading exponents of invertebrate tissue culture from America, Europe, Asia, and Australia.

Contenu

Contributors
Preface
I Application in Medicine
Chapter 1. Arbovirus Studies in Vertebrate Cell Lines
Chapter 2. Tick Tissue Culture and Arboviruses
Chapter 3. Morphogenesis of Sindbis Virus in Cultured Mosquito Cells
Chapter 4. Established Mosquito Cell Lines and the Study of Togaviruses
Chapter 5. Further Studies on the Latent Viruses Isolated from Singh's Aedes Albopictus Cell Line
Chapter 6. Application of Tissue Culture to Problems in Malariology
Chapter 7. Application of Tissue Culture of a Pulmonate Snail to Culture of Larval Shistosoma Mansoni
II Application in Biology
Chapter 8. Insect Cell and Tissue Culture as a Tool for Developmental Biology
Chapter 9. In Vitro Established Lines of Drosophila Cells and Applications in Physiological Genetics
Chapter 10. Metamorphosis of Imaginai Disc Tissue Grown In Vitro from Dissociated Embryos of Drosophila
Chapter 11. Single-Cyst in Vitro Spermatogenesis in Drosophila Hydei
Chapter 12. Role of a Macromolecular Factor in the Spermatogenesis of Silkmoths
Chapter 13. Insect Spermatogenesis In Vitro
Chapter 14. Juvenile Hormone-Included Biosynthesis of Vitellogenin in Organ Cultures of Leucophaeamadera Fat Bodies
Chapter 15. Juvenile Hormone-Induced Vitellogenin Synthesis in Locust Fat Body In Vitro
Chapter 16. In Vitro Analysis of Factors Regulating the Juvenile Hormone Titer of Insects
Chapter 17. In Vitro Action of Ecdysone and Juvenile Hormone on Epidermal Commitment in the Tobacco Hornworm
Chapter 18. Metabolism of Molting Hormone Analogs by Cultured Cockroach Tissues
Chapter 19. Stage and Tissue-Specific Hemoglobin Synthesis in an Invertebrate
Chapter 20. Dissociation and Reaggregation of Fat Body Cells During Insect Metamorphosis
III Nutritional Requirements and Establishment of Cell Lines
Chapter 21. The Development of an Insect Tissue Culture Medium
Chapter 22. Insect Cell Line: Amino Acid Utilization and Requirements
Chapter 23. A Comparison of Amino Acid Utilization by Cell Lines of Culex Tarsal is and of Culex Pipiens
Chapter 24. Utilization of Some Sugars by a Line of Trichoplusia Ni Cells
Chapter 25. Influence of Polyphenol Oxidase on Hemocyte Cultures of the Gypsy Moth
Chapter 26. Effective Colony Formation on Drosophila Cell Lines Using Conditioned Medium
Chapter 27. Comparative Studies with Clones Derived from a Cabbage Looper Ovarian Cell Line, Tn-368
Chapter 28. Growth the Trichoplusia Ni (Tn-368) Cell Line in Suspension Culture
Chapter 29. Effects of Extracts from Echinoderms on Cell Cultures from Mollusksand Echinoderms
Chapter 30. Cytotoxic and Anti Proliferative Subtances in Invertebrates and Poikilothermie Vertebrates
IV Study of Viruses and Protozoa of Agricultural and Forest Importance
Chapter 31. Immunochemical Characterization of the Baculoviruses: Present Status
Chapter 32. In Vitro and in Vivo Comparative Studies of Several Nuclear Polyhedrosis Viruses (NPVS) by Neutralization, Immunofluorescence and Polyacrylamide Gel Electrophoresis
Chapter 33. Characterization of Infectious Components of Autographe Californica Nuclear Polyhedrosis Virus Produced In Vitro
Chapter 34. Utilization of Tissue Culture Techniques to Clone an Insect Cell L I Ne and to Characterize Strains of Baculovirus
Chapter 35. Replication of a Nuclear Polyhedrosis Virus of Choristoneura Fumiferana (Lepidoptera: Tortricidae) in Malacosoma Disstria (Lepidoptera: Lasiocampidae) Hemocyte Cultures
Chapter 36. Replication of Alfalfa Looper Nuclear Polyhedrosis Virus in the Trichoplusia Ni (Tn-368) Cell Line
Chapter 37. An Electron Microscope Study of the Sequence of Events in a Nuclear Polyhedrosis Virus Infection in Cell Culture
Chapter 38. Replication of Amsacta Moorei Entomopoxvirus and Autographa Californica Nuclear Polyhedrosis Virus in Hemocyte Cell Lines from Estigmene Acrea
Chapter 39. Dual Infection of the Trichoplusia Ni Cell Line with the Chilo Iridescent Virus (CIV) and Autographa Californica Nuclear Polyhedrosis Virus
Chapter 40. Propagation of a Microsporidan in a Moth Cell Line