Invertebrate Tissue Culture

Invertebrate Tissue Culture, Volume I, reviews advances in the use of tissue and organ culture in invertebrate research in physiology and pathology. It describes methods in invertebrate tissue culture, including organ culture techniques in liquid and gel media; aseptic rearing of invertebrates for tissue culture; and cell culture of organisms, ranging from Lepidoptera and Diptera to Coleoptera, Orthoptera, Dictyoptera, Hymenoptera, Hemiptera, Crustacea, Arachnida, Merostomacea, and mollusks.
Organized into two sections encompassing 13 chapters, this volume begins with an overview of the general methodology in cell and organ cultures and their preparation from aseptic conditions. It then discusses methods for the examination of cultures, including those concerning ultrastructure studies by electron microscopy. The reader is also introduced to cell cultures obtained from different groups of invertebrates, with emphasis on peculiarities specific to each group; the morphology and physiology of cultured invertebrate cells; and cultivation and growth of cells.
This book is a valuable resource for specialists in the field of invertebrate cell and organ culture.

Contenu

List of Contributors
Preface
Contents of Volume II
I. Methods in Invertebrate Tissue Culture
1 Cell Culture Media and Methods
I. Design of Cell Culture Media
II. Selection of Tissue for Culture
III. Preparation of Tissue for Culture
IV. Cultures in Fluid Medium
V. Vapor Phase
VI. Incubation Temperature
References
2 Organ Culture Methods
I. Introduction
II. Organ Culture Technique in Liquid Medium
III. Organ Culture Methods on a Gel Medium
IV. Conclusions
References
3 Electron Microscopy of Cell and Organ Cultures of Invertebrates
I. Introduction
II. General Techniques and the Adaptation of These Techniques to Invertebrate Tissue Culture
III. Results
IV. Conclusion
References
4 Aseptic Rearing of Invertebrates for Tissue Culture
I. Introduction
II. Sterile Methods for Rearing Invertebrates
III. Conclusions
References
II. Cell Cultures
5 The Morphology and Physiology of Cultured Invertebrate Cells
I. Introduction
II. Sources and Types of Cells Used
III. Morphology of Cells in Primary Cultures
IV. Cell Lines
V. Growth of Cells in Culture
VI. Metabolism of Cultured Cells
References
6 Cell Culture of Lepidoptera
I. Introduction
II. Materials and Techniques
III. Cultivation and Growth of Cells
References
7 Cell Culture of Diptera
I. Introduction
II. Cultures of Drosophila, Calliphora, and Musca Tissues
III. Cultures of Aedes, Culex, Glossina, and Anopheles Tissues
IV. Conclusion
References
8 Cell Culture of Coleoptera, Orthoptera and Dictyoptera
I. Introduction
II. Cell Culture of Coleoptera
III. Cell Culture of Orthoptera
IV. Cell Culture of Dictyoptera
References
9 Cell Culture of Hymenoptera
I. Introduction
II. Culture Media
III. Methods
IV. Cultures Obtained
References
10 Cell Culture of Hemiptera
I. Introduction
II. Leafhopper (Cicadellidae) Cell Culture
III. Aphid (Aphididae) and Planthopper (Delphacidae) Cell Culture
References
11 Cell Cultures of Crustacea, Arachnida, and Merostomacea
I. Introduction
II. Cell Cultures of Crustacea
III. Cell Cultures of Arachnida
IV. Cell Cultures of Merostomacea
References
12 Cell Culture of Mollusks
I. Introduction
II. Techniques for Cell Culture of Mollusks
III. Cell Cultures Obtained
References
13 Cell Culture of Invertebrates Other Than Mollusks and Arthropods
I. Introduction
II. Development of Culture Media
III. Cell Culture of Porifera
IV. Cell Culture of Cnidaria
V. Cell Culture of Platyhelminthes
VI. Cell Culture of Annelids
VII. Cell Culture of Sipunculids
VIII. Cell Culture of Echinoderms
IX. Cell Culture of Stomochordates
X. Cell Culture of Protochordates
References
Author Index
Subject Index