Interaction of Translational and Transcriptional Controls in the Regulation of Gene Expression

Interaction of Translational and Transcriptional Controls in the Regulation of Gene Expression presents the proceedings of the Fogarty International Conference on Translational/Transcriptional Regulation of Gene Expression, held at the National Institutes of Health in Bethesda, Maryland, on April 7-9, 1982. Speakers discussed the molecular strategies at work during the modulation of gene expression following transcriptional initiation. They also discussed recent developments in a number of key areas in which transcriptional and translational components interact.
Organized into five sections encompassing 36 chapters, this volume explores both prokaryotic and eukaryotic systems, as well as structure-function correlations. It begins with an overview of translational/transcriptional controls in prokaryotes, the regulation of gene expression by transcription termination and RNA processing, and the structure and expression of initiation factor genes. It then examines the effect of the codon context on translational fidelity, including mistranslation of messenger RNA; protein synthesis for the construction of cell architecture; regulation of initiation factor activity; and translational regulation in cells.
This book is a valuable resource for Fogarty International Scholars who want to broaden their knowledge and contribute their expertise to the National Institutes of Health community.

Contenu

Preface
Section I: Translational/Transcriptional Controls in Prokaryotes
The Regulation of Gene Expression by Transcription Termination and RNA Processing
Attenuation in the Control of Tryptophan Operon Expression
Organisation and Expression of the E. Coli Phenylalanyl-Transfer RNA Synthetase Operon
Genes for Two E.. Coli Aminoacyl tRNA Synthetases
Structure and Regulation of E. Coli Glutaminyl-tRNA Synthetase
Construction of the Bacteriophage T4 Replication Machine: Regulation of Synthesis of Component Proteins
Control of Translation by the regA Gene of T4 Bacteriophage
Regulation of Ribosome Biosynthesis in Escherichia Coli
Properties of a Yeast tRNAphe Amber Suppressor
Regulation of an Eleven Gene Ribosomal Protein Operon
Ribosomal Protein L10 and SI Control their own Synthesis
Use of Dipeptide Synthesis to Study the In Vitro Expression of the L10 (ß) Operon
The Structure and Expression of Initiation Factor Genes
Regulation of the Expression of tufA and tufB, the Two Genes Encoding the Elongation Factor EF-Tu in E. Coli
Determinants of Translational Specificity in Bacillus subtilis
Section II: Codon Context and Translational Fidelity
Site Directed Mutagenesis of the Anticodon Region: The "Universal U" is not Essential to tRNA Synthesis and Function
Properties of a Yeast tRNAPhe Amber Suppressor
The Effect of Codon Context on Mistranslation of Messenger RNA
Ribosomal Frameshift Errors Control the Expression of an Overlapping Gene in RNA Phage
Section III: Structure and Eukaryotic Translation
Protein Synthesis for the Construction of Cell Architecture
Messenger RNA Conformation and the Regulation of Protein Synthesis
Function and Topography of Components of Initiation Complexes of Eukaryotic Translation
The Protein Translocation Machinery of the Endoplasmic Reticulum
Section IV: Regulation of Initiation Factor Activity
The Control of the Rate of Protein Synthesis Initiation
The Mechanism of Translational Inhibition in Hemin-Deficient Lysates
The Control of Polypeptide Chain Initiation by Phosphorylation of the a Subunit of the Initiation Factor eIF-2
Protein Synthesis Initiation and Ifcs Regulation in Reticulocyte Lysates by eIF-2 and eIF-2-Ancillary Protein Factors
Interactions of Cap Binding Proteins with Eukaryotic mRNAs
Cap Function and Regulation of Translation During Poliovirus Infection
Cap Binding Protein Related Polypeptides in Cytoplasmic Ribonucleoprotein Particles of Chick Embryonic Muscle
The Role of mRNA Competition in Regulating Translation in Normal Fibroblasts
Section V: Translations Regulation in Cells
Mechanisms of Interferon Action: Characteristics of RNase L, the (2'-5')(A)n- Dependent Endoribonuclease
Expression of Human Fibroblast and Human Immune Interferon Genes in Homologous and Heterologous cells
VAI RNA Plays a Role in Adenovirus Late mRNA Translation
Some Aspects of Metabolic Regulation of Translation in Cultured Eukaryotic Cells
Fertilization Triggers Rapid Sequence-Specific Changes in the Translational Activities of Individual Messenger RNAs
Heat Shock in Saccharomyces Cerevisiae: Quantitation of Transcriptional and Translational Effects
Concluding Remarks
Index