Heme, Chlorophyll, and Bilins

Although researchers can profitably investigate heme, chlorophyll, and related tetrapyrroles in a wide range of academic and medical research programs, the handling and manipulation of these delicate compounds requires considerable skill and cross-boundary knowledge. In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators overcomes these limitations by describing in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles, heme, and hemoproteins, to the biosynthesis and the analysis of chlorophyll and bilins.
Timely and highly practical, Heme, Chlorophyll, and Bilins: Methods and Protocols is a gold-standard collection of readily reproducible techniques suitable for a wide range of researchers, whether it be a clinician studying photodynamic therapy, an ecologist studying the chlorophyll composition of leaves in a tropical forest, or a cell biologist investigating the function of specific hemoproteins.

Texte du rabat

In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators describe in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles,heme, and hemoproteins, to the biosynthesis and analysis of chlorophyll and bilins.

Contenu

Laboratory Methods for the Study of Tetrapyrroles Alison G. Smith and Michael Witty Syntheses of Tetrapyrroles Kevin M. Smith General Laboratory Methods for Tetrapyrroles Jerry C. Bommer and Peter Hambright Enzymatic Preparation of Tetrapyrrole Intermediates Martin J. Warren and Peter M. Shoolingin-Jordan Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme Chang Kee Lim Analysis of Intermediates and End Products of the Chlorophyll Biosynthetic Pathway Constantin A. Rebeiz Analysis of Heme and Hemoproteins Angela Wilks Hemoproteins Purification and Characterization by Using Aqueous Two-Phase Systems Daniel Forciniti Structural Study of Heme Proteins by Electron Microscopy of 2-Dimensional Crystals Terrence G. Frey Analysis and Reconstitution of Chlorophyll-Proteins Harald Paulsen and Volkmar H.R. Schmid Two-Dimensional Crystallization of Chlorophyll-Proteins Georgios Tsiotis Biosynthesis and Analysis of Bilins Matthew J. Terry Analysis and Reconstitution of Phytochromes Michael T. McDowell and J. Clark Lagarias Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions Wendy M. Schluchter and Donald A. Bryant