Lipolytic Enzymes

Lipolytic Enzymes focuses on the biochemistry of lipolytic enzymes, particularly, pancreatic lipase and phospholipase 2 as well as their structure and catalytic mechanism. It explores the kinetics of lipolysis, the digestive lipases of nonmammalian animals, the assay and purification of cholesterol esterases and phospholipases, the method phospholipases use in hydrolyzing phospholipids, and the adaptive mechanism of lipolytic enzymes at the lipid-water interface.
Organized into eight chapters, this book begins with an overview of the importance of lipolytic enzymes, including their medical, therapeutic, food, and other industrial applications. It then proceeds with a discussion on the classification of lipolytic enzymes according to the type of bond they hydrolyze and the substrates on which they act. The next chapters look at the substrates and supersubstrates of lipolytic enzymes, along with their maximal velocity and the Michaelis constant. Moreover, the book talks about the detection and assay of lipases, the molecular properties of pancreatic cholesterol esterases, the stimulating effect of bile salts on cholesterol esterases, the hydrolytic cleavage of carboxyl esterases, and the occurrence and distribution of phosphohydrolases. A chapter discussing the two groups of lipolytic enzymes (the first containing enzymes of broad substrate specificity and not requiring cofactors; the second containing metalloenzymes with very narrow substrate requirements) concludes this book.
This book is a valuable resource for chemists, biochemists, and those working in the field of nutritional sciences.

Contenu

Preface
Abbreviations and Definitions
I. Introduction
Importance of Lipolytic Enzymes
II. Nomenclature
I. Substrates
II. Table of Lipolytic Enzymes
III. Kinetics of Lipolysis
I. Substrate and Supersubstrate
II. Maximal Velocity
III. Determination of the Km
IV. Meaning of Km
V. Inhibition at the Interface
VI. pH and Cations at the Interface
VII. Monolayers
VIII. Water-Soluble Substrates
IV. Lipases
I. Detection and Assay
II. Pancreatic Lipase
III. Gastric, Pregastric, and Lingual Lipases
IV. Digestive Lipases of Nonmammalian Animals
V. Lipoprotein Lipase
VI. Tissue Lipases
VII. Milk Lipases
VIII. Plant Lipases
IX. Microbial Lipases
X. Monoglyceride Lipases
XI. Glycosyldiglyceride Lipases
V. Cholesterol Esterase
I. Assay
II. Purification
III. Molecular Properties of Pancreatic Cholesterol Esterases
IV. Substrate Specificity
V. Inhibitors
VI. Requirement for Bile Salts
VII. Intracellular Cholesterol Esterases
VIII. Conclusion
VI. Phospholipases: Carboxyl Esterases
I. Phospholipase 2
II. Phospholipase 1
III. Lysophospholipase
VII. Phospholipases: Phosphohydrolases
I. Phosphatidate Phosphohydrolase
II. Phospholipase 3
III. Sphingomyelinase
IV. Phospholipase 4; Ceramidase
VIII. Synopsis
I. The Nonspecific Lipolytic Enzymes
II. Metal-Dependent Lipolytic Enzymes
III. Nature of Lipolytic Enzymes
Bibliography
Subject Index