Bioconjugate Techniques

Bioconjugate Techniques, Third Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

• Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
• Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
• Features full color illustrations
• Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Texte du rabat

Bioconjugate Techniques, Third Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

• Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
• Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
• Features full color illustrations
• Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Contenu

1. Introduction to Bioconjugation 2. Functional Targets for Bioconjugation 3. The Reactions of Bioconjugation 4. Zero-Length Crosslinkers 5. Homobifunctional Crosslinkers 6. Heterobifunctional Crosslinkers 7. Trifunctional Crosslinkers 8. Dendrimers and Dendrons 9. Cross-Bridges and Cleavable Reagent Systems 10. Fluorescent Probes 11. (Strept)avidin - Biotin Systems 12. Isotopic Labeling Techniques 13. Silane Coupling Agents 14. Microparticles and Nanoparticles 15. Immobilization of Ligands on Chromatography Supports 16. Buckyballs, Fullerenes, and Carbon Nanotubes 17. Chemoselective Ligation; Bioorthogonal Reagents 18. PEGylation & Synthetic Polymer Modification 19. Vaccine and Immunogen Conjugates20. Antibody Modification and Conjugation 21. Liposome Conjugates and Derivatives 22. Enzyme Modification and Conjugation 23. Nucleic Acid and Oligonucleotide Modification and Conjugation 24. Bioconjugation for the Study of Protein Interactions