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Methods in Neurosciences, Volume 1: Gene Probes is a compendium of papers that deals with the developments in molecular biology, cell biology, and electrophysiology. Section I deals with gene expression using the Xenopus Oocyte system for expression and cloning of neuroreceptors and channels. One paper presents a method in studying the molecules in the brain related to neurotransmitter receptors and to the voltage channels in the brain by ""transplanting"" functional neurotransmitter receptors into the membrane of frog oocytes. Section II describes in situ and solution hybridization and the continuation of in situ hybridization with immunohistochemistry.
One paper discusses the benefits of using alkaline phosphatase-Dig-dUTP-labeled oligonucleotide probes in high-resolution in situ in terms of ease of performance, safety, and fast detection rendering. Section III addresses the screening, sequencing, and cloning, the process of which includes the rapid identification of DNA clones. One paper outlines the methods and materials that are used in such identification. Section IV explains lineage analysis and Section V discusses molecular pathology, including the molecular pathology of Alzheimer's disease.
This collection can prove useful for geneticists, molecular scientists, and academicians involved in neuroscience and pharmacological sciences, as well as researchers in geriatrics.
Contenu
Section I Gene Expression
Chapter 1: Using the Xenopus Oocyte System for Expression and Cloning of Neuroreceptors and Channels
Chapter 2: Expression of Neurotransmitter Receptors and Voltage-Activated Channels from Brain mRNA in Xenopus Oocytes
Chapter 3: Expression of Mammalian Plasma Membrane Receptors in Xenopus Oocytes: Studies of Thyrotropin-Releasing Hormone Action
Chapter 4: Expression of Exogenous Voltage-Gated Calcium Channels in Xenopus Oocytes
Section II In Situ and Solution Hybridization
Chapter 5: Quantification of in Situ Hybridization Histochemistry for Analysis of Brain Function
Chapter 6: In Situ Hybridization: A Methodological Guide
Chapter 7: Combination of in Situ Hybridization with Immunohistochemistry and Retrograde Tract-Tracing
Chapter 8: Localization of Neuronal mRNAs by Hybridization Histochemistry
Chapter 9: In Situ Detection of Peptide Messenger RNA Using Complementary RNA Probes
Chapter 10: Quantification of mRNA in Discrete Cell Groups of Brain by in Situ Hybridization Histochemistry
Chapter 11: In Situ Hybridization Approaches to Human Neurological Disease
Chapter 12: Localization of Peptide Gene Expression by in Situ Hybridization at Electron Microscopic Level
Chapter 13: In Situ Hybridization for Detecting Gonadotropin-Releasing Hormone Messenger RNA and Measuring Physiologically Stimulated Changes
Chapter 14: Location of Gene Expression in Tissue Sections by Hybridization Histochemistry Using Oligodeoxyribonucleotide Probes
Chapter 15: In Situ mRNA Hybridization: Standard Procedures and Novel Approaches
Chapter 16: Nonradioactive in Situ Hybridization Histochemistry with DigoxigeninâEUR"Deoxyuridine 5âEUR²-Triphosphate-Labeled Oligonucleotides
Chapter 17: Quantitation of Nuclear Low-Level Gene Expression in Central Nervous System Using Solution Hybridization and in Situ Hybridization
Section III Screening, Sequencing, and Cloning
Chapter 18: Immunoscreening λgt11 cDNA Library
Chapter 19: Rapid Identification of DNA Clones: Utilization of Same Degenerate Oligonucleotides for Both Screening and Sequencing
Chapter 20: Molecular Cloning of Nicotinic Acetylcholine Receptor Genes
Section IV Lineage Analysis
Chapter 21: Lineage Analysis in the Vertebrate Nervous System by Retrovirus-Mediated Gene Transfer
Chapter 22: Use of Transgenic Models to Access Neural Lineages in Mammals
Section V Molecular Pathology
Chapter 23: Characterization of Molecular Pathology of AlzheimerâEUR(TM)s Disease
Section VI Appendixes
Appendix I: Restriction Endonuclease Sites of Cleavage
Appendix II: Amino Acids and Genetic Code
Index