Enzymology in the Practice of Laboratory Medicine

Enzymology in the Practice of Laboratory Medicine focuses on disease processes, enzymatic reactions and activities, and diagnosis and management of diseases.

The selection first offers information on developments in the search for mechanisms of enzymic catalysis; application of the spin labeling technique to biological membranes; and the properties of membrane bound enzymes involved in energy transduction processes. Discussions focus on possible clinical interrelationships of membrane function, mitochondrial membrane transactions, mechanical details of chymotryptic catalysis, and physical nature of globular proteins. The text then elaborates on metalloenzymes, enzyme measurement, and enzymology, clinical chemistry, and development of fast analyzers.

The publication examines the analytical uses of immobilized enzymes; origin of serum enzymes and the basis for their variation; and the screening methods for glycolytic enzyme deficiencies in red blood cells. Topics include factors contributing to normal variation of enzyme activity, catabolism of enzymes, mechanism of release from cells to plasma, and determination of urea in blood and urine.

The selection is a dependable reference for researchers interested in the application of enzymology in laboratory medicine.

Contenu

Contributors and Participants

Preface

Molecular Biology

Some Recent Developments in the Search for Mechanisms of Enzymic Catalysis

Application of the Spin Labeling Technique to Biological Membranes

The Properties of Membrane Bound Enzymes Involved in Energy Transduction Processes

Metalloenzymes

Analytical Methodology

Some Thoughts on Enzyme Measurement

Enzymology, Clinical Chemistry and the Development of Fast Analyzers

Analytical Uses of Immobilized Enzymes

Experiences with the GeMSAEC Fast Analyzer at the University of Minnesota

Pathophysiology and Clinical Interpretation of Laboratory Data

The Origin of Serum Enzymes and the Basis for Their Variation

Screening Methods for Glycolytic Enzyme Deficiencies in Red Blood Cells

Alpha-1-Antitrypsin

Fabry's Disease

Clinical Significance of the Determination of Isoenzyme Activities

Serum Creatine Phosphokinase and Serum Aldolase Levels in Acutely Psychotic Patients

Clinical Enzymology in the Evaluation of Heart and Liver Disease

Multivariate Clustering