A New Look at Mechanisms In Bioenergetics

A New Look at Mechanisms in Bioenergetics features eight lectures based on the Robbins Lectures given at Pomona College in April 1973. These lectures are based mainly on the author's own laboratory work and are intended for students of biology and biochemistry who want to devote their lives to research.
Lecture 1 presents some of the general lessons learned from research in the field of bioenergetics. It also discusses methods for measuring oxidative phosphorylation and the resolution of soluble multienzyme systems. Lecture 2 explains the biochemical approach to the problem of photophosphorylation. Lecture 3 considers the intersection of oxidative phosphorylation and membranology. Lecture 4 discusses the coupling device and its partial reactions. Lecture 5 focuses on the oxidation chain in mitochondria. Lecture 6 discusses the resolution and reconstitution of oxidative phosphorylation. Lecture 7 examines the reconstitution of ion pumps. Finally, Lecture 8 covers oxidation control in glycolysis; the high aerobic glycolysis of tumor cells; ATPases in tumor cells; and the repair of ion pumps in tumor cells.

Contenu

Preface
Abbreviations
Lecture 1 Troubles Are Good for You
How It All Started
What Is Oxidative Phosphorylation?
How Do We Measure Oxidative Phosphorylation?
First Approaches to the Resolution of the Membrane
Alio topic Properties of F1
Electron Microscopy
Isolation of Resolved Particles
Reconstitution of Oxidative Phosphorylation
Lecture 2 Photophosphorylation
On the Origin of Life
Electron Pathway in Photophosphorylation
Further Similarities with Mitochondria
Resolution of a Coupling Factor
Proton Movements in Chloroplasts
Asymmetric Assembly of the Chloroplast Membrane
Reversal of Photophosphorylation
Lecture 3 Functions and Structure of Membranes and the Mechanism of Phosphorylation Coupled to Electron Transport
General Comments
Function and Structure of Membranes
Mechanism of Phosphorylation Coupled to Electron Transport
Experimental Approaches
Lecture 4 The Coupling Device
Partial Reactions and Components of the Coupling Device
The ATP-Driven Proton Pump
Lecture 5 The Oxidation Chain and the Topography of the Inner Mitochondrial Membrane
Analysis of the Oxidation Chain
Isolation of Complexes and Individual Catalysts
The Three Segments of the Oxidation Chain
The Topography of the Oxidation Chain
Lecture 6 Resolution and Reconstitution of Oxidative Phosphorylation
General Comments
Reconstitutions of Mitochondrial and Chloroplast Membrane Functions
Reconstitution of the Proton Pump of Halobacterium halobium and of Rhodopsin-Catalyzed Photophosphorylation
Lecture 7 Reconstitution and Mechanism of Action of Ion Pumps
General Comments
Reconstitution of Ion Pumps
What Can We Learn from Resolution and Reconstitution Experiments?
Lecture 8 Control of Energy Metabolism
Oxidation Control
The Pasteur Effect
The Competition Mechanism
High Aerobic Glycolysis in Tumor Cells (The Warburg Effect)
ATPases in Tumor Cells
Repair of Ion Pumps in Tumor Cells
Bibliography
Index