Cyanobacteria Biotechnology

Guides the reader through the interesting and useful aspects of cyanobacteria metabolism but will keep true to a biotechnology vision.

Das E-Book Cyanobacteria Biotechnology wird angeboten von Wiley-VCH GmbH und wurde mit folgenden Begriffen kategorisiert:

Biochemical Engineering, Biochemische Verfahrenstechnik, Biotechnologie, Biotechnologie i. d. Biowissenschaften, Biotechnology, Biowissenschaften, Chemical Engineering, Chemische Verfahrenstechnik, Cyanobakterien, Life Sciences, Microbiology & Virology, Mikrobiologie, Mikrobiologie u. Virologie

Auteur
Paul Hudson is an Associate Professor (2018) of Metabolic Engineering in the School of Engineering Sciences in Chemistry, Biotechnology, and Health at the Royal Institute of Technology (KTH) in Stockholm Sweden. He has a Ph.D. degree in Chemical Engineering from U.C. Berkeley (2009). He has published 26 research papers in the fields of protein science, microbial metabolic engineering, and systems biology. The main focus of his research is on systems and synthetic biology of cyanobacteria.

Résumé

Unites a biological and a biotechnological perspective on cyanobacteria, and includes the industrial aspects and applications of cyanobacteria

Cyanobacteria Biotechnology offers a guide to the interesting and useful features of cyanobacteria metabolism that keeps true to a biotechnology vision. In one volume the book brings together both biology and biotechnology to illuminate the core acpects and principles of cyanobacteria metabolism.

Designed to offer a practical approach to the metabolic engineering of cyanobacteria, the book contains relevant examples of how this metabolic "module" is currently being engineered and how it could be engineered in the future. The author includes information on the requirements and real-world experiences of the industrial applications of cyanobacteria. This important book:

• Brings together biology and biotechnology in order to gain insight into the industrial relevant topic of cyanobacteria
• Introduces the key aspects of the metabolism of cyanobacteria
• Presents a grounded, practical approach to the metabolic engineering of cyanobacteria
• Offers an analysis of the requirements and experiences for industrial cyanobacteria
• Provides a framework for readers to design their own processes
  Written for biotechnologists, microbiologists, biologists, biochemists, Cyanobacteria Biotechnology provides a systematic and clear volume that brings together the biological and biotechnological perspective on cyanobacteria.

Contenu

Foreword: Cyanobacteria Biotechnology xv

Acknowledgments xviii

Part I Core Cyanobacteria Processes 1

1 Inorganic Carbon Assimilation in Cyanobacteria: Mechanisms, Regulation, and Engineering 3
*Martin Hagemann, Shanshan Song, and Eva-Maria Brouwer*

1.1 Introduction The Need for a Carbon-Concentrating Mechanism 3

1.2 The Carbon-Concentrating Mechanism (CCM) Among Cyanobacteria 4

1.2.1 Ci Uptake Proteins/Mechanisms 5

1.2.2 Carboxysome and RubisCO 8

1.3 Regulation of Ci Assimilation 10

1.3.1 Regulation of the CCM 10

1.3.2 Further Regulation of Carbon Assimilation 13

1.3.3 Metabolic Changes and Regulation During Ci Acclimation 14

1.3.4 Redox Regulation of Ci Assimilation 15

1.4 Engineering the Cyanobacterial CCM 16

1.5 Photorespiration 17

1.5.1 Cyanobacterial Photorespiration 17

1.5.2 Attempts to Engineer Photorespiration 19

1.6 Concluding Remarks 20

Acknowledgments 21

References 21

2 Electron Transport in Cyanobacteria and Its Potential in Bioproduction 33
*David J. Lea-Smith and Guy T. Hanke*

2.1 Introduction 33

2.2 Electron Transport in a Bioenergetic Membrane 34

2.2.1 Linear Electron Transport 34

2.2.2 Cyclic Electron Transport 37

2.2.3 ATP Production from Linear and Cyclic Electron Transport 37

2.3 Respiratory Electron Transport 38

2.4 Role of Electron Sinks in Photoprotection 41

2.4.1 Terminal Oxidases 41

2.4.2 Hydrogenase and Flavodiiron Complexes 41

2.4.3 Carbon Fixation and Photorespiration 43

2.4.4 Extracellular Electron Export 44

2.5 Regulating Electron Flux into Different Pathways 45

2.5.1 Electron Flux Through the Plastoquinone Pool 45

2.5.2 Electron Flux Through Fdx 46

2.6 Spatial Organization of Electron Transport Complexes 47

2.7 Manipulating Electron Transport for Synthetic Biology Applications 48

2.7.1 Improving Growth of Cyanobacteria 49

2.7.2 Production of Electrical Power in BPVs 49

2.7.3 Hydrogen Production 50

2.7.4 Production of Industrial Compounds 50

2.8 Future Challenges in Cyanobacterial Electron Transport 51

References 52

3 Optimizing the Spectral Fit Between Cyanobacteria and Solar Radiation in the Light of Sustainability Applications 65
*Klaas J. Hellingwerf, Que Chen, and Filipe Branco dos Santos*

3.1 Introduction 65

3.2 Molecular Basis and Efficiency of Oxygenic Photosynthesis 67

3.3 Fit Between the Spectrum of Solar Radiation and the Action Spectrum of Photosynthesis 72

3.4 Expansion of the PAR Region of Oxygenic Photosynthesis 74

3.5 Modulation and Optimization of the Transparency of Photobioreactors 79

3.6 Full Control of the Light Regime: LEDs Inside the PBR 81

3.7 Conclusions and Prospects 82

References 83

Part II Concepts in Metabolic Engineering 89

4 What We Can Learn from Measuring Metabolic Fluxes in Cyanobacteria 91
*Xiang Gao, Chao Wu, Michael Cantrell, Melissa Cano, Jianping Yu, and Wei Xiong*

4.1 Central Carbon Metabolism in Cyanobacteria: An Overview and Renewed Pathway Knowledge 91

4.1.1 Glycolytic Routes Interwoven with the Calvin Cycle 91

4.1.2 Tricarboxylic Acid Cycling 94

4.2 Methodologies for Predicting and Quantifying Metabolic Fluxes in Cyanobacteria 95

4.2.1 Flux Balance Analysis and Genome-Scale Reconstruction of Metabolic Network 95

4.2.2 13C-Metabolic Flux Analysis 96

4.2.3 Thermodynamic Analysis and Kinetics Analysis 99

4.3 Cyanobacteria Fluxome in Response to Altered Nutrient Modes and Environmental Conditions 101

4.3.1 Autotrophic Fluxome 101 <p&...