Catalytic RNA

In recent years, unprecedented advances in many aspects of the molecular biology of nucleic acids have been witnessed. The area of RNA chemistry has undergone a kind of explosion, with a huge interest in RNA-mediated catalysis. At the same time, our structural understanding of DNA-protein interactions has increased enormously, and the related area of RNA-protein interactions is beginning to gather pace. This softcover edition from the successful series Nucleic Acids and Molecular Biology is devoted to the structure and mechanism of ribozymes, and their potential exploitation. The subject has both important evolutionary implications and potential practical application in the development of therapeutic agents for diseases such as AIDS.

Contenu

Group I Ribozymes: Substrate Recognition, Catalytic Strategies and Comparative Mechanistic Analysis.- Dynamics of a Group I Ribozyme Detected by Spectroscopic Methods.- The Structure of Group I Ribozymes.- Interaction of Aminoglycoside Antibiotics with RNA.- Catalytic Reaction Mechanisms and Structural Features of Group II Intron Ribozymes.- Structural Analysis of the Bacterial Ribonuclease P RNA.- Structural Analysis and Modifications of the Hairpin Ribozyme.- Circular RNAs: Generation of Small RNAs with Unique Properties by Splicing Permuted Intron-Exon Sequences.- Three-Dimensional Structure of the Hammerhead Ribozyme.- The Hammerhead Ribozyme.- Probing the Cleavage Activity of the Hammerhead Ribozyme Using Analog Complexes.- Mechanistic Studies on Hammerhead Ribozymes.- Hammerhead Ribozymes with Long Flanking Sequences: a Structural and Kinetic View.- Design, Synthesis and Function of Therapeutic Hammerhead Ribozymes.- Synthetic Catalytic Oligonucleotides Based on the Hammerhead Ribozyme.- Efficacy of Hammerhead Ribozymes Targeting ?-Lactalbumin Transcripts: Experiments in Cells and Transgenic Mice.- Anti-HIV Therapeutic Hammerhead Ribozymes: Targeting Strategies and Optimization of Intracellular Function.- Inhibition of HIV Replication by the Hairpin Ribozyme.- The Use of Ribozymes to Inhibit HIV Replication.- RNA Catalysis in Fungi.- In Vitro Selection of Catalytic RNA.- RNA Catalysis and Transition State Stabilization.- Subject Index for Volume 10.- Author Index for Volumes 6-10.- Subject Index for Volumes 6-10.