Biology of Methylotrophs

Biology of Methylotrophs introduces the reader to the study of methylotrophs - microorganisms that use reduced one-carbon compounds for growth.
The book is divided into five parts. Part I covers the taxonomy, morphology, and ultrastructure of methylotrophic bacteria. Part II discusses the processes involved in their growth and metabolism. Part III talks about the possible applications of methylotrophs and their enzymes in industrial fields as well as chemistry. Part IV deals with the molecular genetics and the gene expression of methylotrophs, and Part V deals with their habitat and role in the environment.
The text is recommended for microbiologists who would like to be acquainted with the subject or make further studies about methylotrophs.

Contenu

Preface
Introduction
Part I. Taxonomy and Morphology

1. Taxonomy of Methylotrophic Bacteria
   1.1 The Methane-Oxidizing Bacteria
   1.2 Obligate and Restricted Facultative Methanol-Utilizing Bacteria
   1.3 Facultative Methylotrophic Bacteria
   1.4 Relationship of Methylotrophic Bacteria Taxa with Other Prokaryotic Taxa
   1.5 A New Classification Scheme for the Methylotrophs
   References
2. Systematics of Methylotrophic Yeasts
   2.1 General Characteristics of Methanol-Utilizing Yeasts
   2.2 Methane-Utilizing Yeasts
   2.3 Applications of Methanol-Utilizing Yeasts
   2.4 Conclusion
   References
3. Ultrastructure of Methylotrophic Microorganisms
   3.1 Prokaryotic Cells
   3.2 Eukaryotic Microbes
   References
   Part II. Growth and Metabolism
4. Assimilation of Carbon by Methylotrophs
   4.1 The Ribulose Bisphosphate (RuBP) Pathway
   4.2 The Ribulose Monophosphate (RuMP) Pathway
   4.3 The Dihydroxyacetone (DHA) Cycle of Formaldehyde Assimilation in Yeasts
   4.4 The Serine Pathway of Formaldehyde Assimilation 101
   4.5 Distribution and Occurrence of the Assimilation
   Pathways
   References
5. Oxidation Pathways in Methylotrophs
   5.1 Bacterial Oxidation of C1 Compounds
   5.2 Oxidation of C1 Compounds in Yeasts
   5.3 Conclusions and Biotechnological Implications
   References
6. Regulation of Oxidation and Assimilation of One-Carbon Compounds in Methylotrophic Bacteria
   6.1 Regulation of the Oxidation of C1 Compounds in Bacteria
   6.2 Regulation of the Assimilation of C1 Compounds in Bacteria
   References
7. Growth Yields, Productivities, and Maintenance Energies of Methylotrophs
   7.1 Basic Bioenergetic Concepts
   7.2 Aerobic Growth and Product Formation
   7.3 Anaerobic Growth and Product Formation
   7.4 Conclusions
   References
8. Mixed Substrates and Mixed Cultures
   8.1 Introduction
   8.2 Methylotrophic Bacteria
   8.3 Methylotrophic Yeasts
   References
   Part III. Uses of Methylotrophs
9. Single Cell Protein Production from C1 Compounds
   9.1 Overview of Single Cell Protein Use
   9.2 Industrial SCP Production Using C1 Compounds
   9.3 Is There a Future for SCP Production?
   References
10. Enzymes of Industrial Potential from Methylotrophs
    10.1 Hydrolases and Lyases
    10.2 Oxidoreductases
    10.3 Perspectives
    References
11. Production of Useful Chemicals by Methylotrophs
    11.1 Production of Chemicals by C1-Oxidative Enzymes
    11.2 Production of Respiratory Coenzymes
    11.3 Glycerol Production in Yeast
    11.4 Polyol Production in Yeast
    11.5 Amino Acid Production
    11.6 Poly-ß-Hydroxybutyric Acid Production
    11.7 Production of Other Metabolites
    11.8 Conclusions
    References
    Part IV. Genetics
12. Molecular Genetics of Methylotrophic Bacteria
    12.1 General Molecular Genetics
    12.2 Mutagenesis
    12.3 Surrogate and Classical Genetics
    12.4 Gene Cloning in the Methylotrophs
    12.5 Regulation and Genetics of Amino Acid Biosynthesis
    12.6 Genes Involved in C1 Oxidation and Assimilation
    References
13. Heterologous Gene Expression in Methylotrophic Yeast
    13.1 Vectors and Hosts Used for Heterologous Gene Expression
    13.2 Expression of Heterologous Proteins in the Cytoplasm
    13.3 Expression and Secretion of Heterologous Proteins
    13.4 Intracellular Transport and Post-Translational Modifications
    13.5 Discussion
    References
    Part V. Ecology
14. Ecology of Methylotrophic Bacteria
    14.1 Role of Methane in the Biosphere
    14.2 Ecological Importance of Soluble Methane Monooxygenase in Methanotrophic Bacteria
    14.3 Methods for Estimation of Populations of Methane-Utilizing Bacteria in Soil and Water Samples
    14.4 Methane Production and Oxidation in Aquatic Ecosystems
    14.5 Distribution of Methane-Oxidizing Bacteria in Freshwater Environments
    14.6 Methanotrophic Bacteria in Seawater
    14.7 Methane Utilization in Soils
    14.8 Are Soils and Aquatic Environments Sinks for Atmospheric Methane?
    14.9 Fortuitous Metabolism of Methanotrophs and Its Environmental Significance
    14.10 Anaerobic Methane Oxidation
    14.11 Ecology and Diversity of Bacteria That Grow on C1 Substrates Other Than Methane
    References