Biological Applications of Magnetic Resonance

Biological Applications of Magnetic Resonance discusses various applications of magnetic resonance techniques. The book's opening chapter examines the exchange behavior of the hydrogen-bonded protons and its influence on their nuclear magnetic resonance (NMR) spectra. This is followed by separate chapters dealing with NMR studies of nucleic acid and drug-nucleic acid complexes. Aspects of the basic theory of the nuclear Overhauser effect (NOE) are presented, along with applications of NOEs observed for protons in biomolecules. Subsequent chapters cover specialized EPR techniques that have been applied to biological problems; the use of physical methods to refine a model of the combing site of the Fv fragment of protein 315; and the utility of model compounds in the analysis of hemoprotein NMR spectra. The remaining chapters discuss serine proteinases that have been investigated by nuclear magnetic resonance spectroscopy; the use of NMR in the study of intact living tissue and organs; and 31P and 13C NMR studies of E. coli cells.

Contenu

Contributors
Chapter 1. Hydrogen-Bonded Proton Exchange and Its Effect on NMR Spectra of Nucleic Acids
Chapter 2. Elucidation of Nucleic Acid Structure by Proton NMR
Chapter 3. Nuclear Magnetic Resonance Studies of Drug-Nucleic Acid Complexes
Chapter 4. Nuclear Overhauser Effects on Protons, and Their Use in the Investigation of Structures of Biomolecules
Chapter 5. Pulsed EPR Studies of Metalloproteins
Chapter 6. An Antibody Building Site: A Combined Magnetic Resonance and Crystallographic Approach
Chapter 7. Model Compounds as Aids in Interpreting NMR Spectra of Hemoproteins
Chapter 8. Multinuclear NMR Approaches to the Solution Structure of Alkaline Phosphatase: 13C, 19F, 31P, and 113Cd NMR
Chapter 9. Catalytic Groups of Serine Proteinases: NMR Investigations
Chapter 10. 31P NMR in Living Tissue: The Road from a Promising to an Important Tool in Biology
Chapter 11. High Resolution 31P and 13C Nuclear Magnetic Resonance Studies of Escherichia coli Cells in Vivo
Index