Biochemistry of Membrane Transport

This volume contains the proceedings of the FEBS Sym­ posium on the Biochemistry of Membrane Transport, which was held at the Swiss Institute of Technology, Zlirich, July 18-23, 1976. Of the speakers invited or­ iginally, only five could not attend the meeting, and of the lectures given, all but one of the texts are published here. Thus, this volume gives a faithful ac­ count of the way the meeting was originally conceived and actually took place. This Symposium on Biochemistry of Membrane Transport was the first Symposium sponsored by the FEBS outside the yearly FEBS-Meetings, after the Special Meeting on Industrial Biochemistry, which took place in Dublin in 1973, and it reflects the interest and the trend for gatherings of smaller size than the official FEBS Meetings. The topic of the Symposium was an easy choice, not only because membrane transport is becom­ ing more and more important to biochemistry every year, but also because of the long-standing interest of Swiss Science in the field. In the choice of the topics and of the speakers, efforts were made to achieve as balanced a coverage of the area as possible. However, since some aspects of membrane biochemistry were dealt with extensively at the parallel 10th Inter­ national Congress of Biochemistry in Hamburg, GFR, cer­ tain topics were given less emphasis than others. The Symposium was attended by about 400 participants (we expected 200-250); among them 48 were invited speakers, and some 200 contributed posters.

Contenu

Electron Microscopy of Membranes.- Electron Microscopic Studies on Membranes.- Motional Freedom of Integral Proteins in the Mitochondrial Energy-Transducing Membrane.- Lipid-Lipid and Lipid-Protein Interactions in Membranes.- Some Aspects of Lipid-Phase Transition on Membrane Permeability and Lipid-Protein Association.- A Calorimetric and Fluorescent Probe Study of Phase Transitions in Phosphatidylcholine Liposomes.- Phase Transitions, Protein Aggregation and a New Method for Modulating Membrane Fluidity.- Rotational Diffusion of Proteins in Membranes.- Protein-Protein Interactions in the Purple Membrane.- Incorporation of Pure Hydrolases Isolated from Brush Border Membranes in Single-Bilayer Lecithin Vesicles.- Ionophores.- Chiral Recognition of Biogenic Amines by Synthetic Neutral Ionophores in Membranes.- Recent Developments in the Structure-Functional Studies of Peptide Ionophores.- Elementary Steps and Dynamic Aspects of Carrier-Mediated Cation Transport Through Membranes: the Streptogramin Antibiotics (Group B).- Studies on the Gramicidin Channel.- Kinetic Aspects of Membrane Transport.- The Asymmetry of Sugar Transport in the Red Cell Membrane.- Nonlinearity of Reciprocal Plots in Transport Kinetics.- Transport in Epithelia and in Some Other Mammalian Cells.- Hydrogen-Ion Dissociation as a Factor in Amino Acid Transport.- Electrogenic Ion Pump as an Energy Source for Active Amino Acid Transport in Ehrlich Cells.- Some Common Aspects of Active Solute Transport in Yeast and Mouse Ascites Tumour Cells.- Reconstitution of Na+ Gradient-Coupled Carrier Functions of Brush Border Membranes of Intestine and Kidney in Sonicated Liposomes.- A Possible Common Activation Site in Na+-Driven Transport Systems. Partial Purification of the Na-Dependent D-Glucose Transport System From Small Intestinal Brush Borders.- Amino Acid Transport and Binding Activity from Membrane Preparations of Animal Cells.- Sidedness and Coupling of Transport Processes in Small Intestinal and Renal Epithelia.- Transport in Erythrocyte Membranes.- The Action of 2-(4?-Amino Phenyl)-6-Methyl Benzene Thiazol-3,7?-Disulfonic Acid (APMB) on Anion Transport and the Protein in Band 3 of the Red Blood Cell Membrane.- NAP-Taurine, a Photoaffinity Probe for the Anion Transport System of the Red Blood Cell.- Structural and Functional Properties of the Anion Transport System Isolated from Human Erythrocyte Membranes.- Reconstitution and Purification of the D-Glucose Transport Protein from Human Erythrocytes.- Transport ATPases.- Titration of Sodium Against Potassium by Their Action on (Na+, K+) Transport ATPase.- Concept on the Link Between Chemical and Electro-Chemical Free Energies in (NaK)-ATPase Transport Function.- Purification and Properties of Na, K-ATPases from the Rectal Gland of Squalus acanthias and the Electric Organ of Electrophorus electricus and Reconstitution of the Na+-K+ Pump from the Purified Enzyme.- Some Aspects of the Ca-Pump in Human Red Blood Cells.- Protein-Protein Interactions and the Functional States of Sarcoplasmic Reticulum Membranes.- Energy Interconversion in Sarcoplasmic Membranes.- Transport of Ca2+ in Excitable and Other Cells.- Calcium and the Control of Neurosecretion.- The Molecular Organisation of the Sodium Channels in Nerve.- Voltage Clamp Analysis of Catecholamine-Induced Calcium Conductance Changes in Mammalian Cardiac Muscle.- Regulation of Transport Processes.- Effect of Cyclic-Adenosine-Monophosphate on Chloride Transport Across some Mammalian Epithelia.- Steroid Hormone-Induced Alterations in Membrane Lipids - the Basis for Altered Ion Transport.- Calcium, Glucose Transport and Insulin Action.- Ion Transport in Mitochondria.- The Proton Pump of the Mitochondrial Respiratory Chain.- The Stoichiometric Coupling of H+ Efflux During Electron Transport to H+ Influx During ATP Synthesis by Mitochondria.- Mitochondrial Calcium Transport and the Intracellular Calcium Homeostasis.- In vivo Measurements of Ionized Mg2+ and Ca2+ in Single Cells.- The ADP, ATP Translocation and its Catalyst.- Bacterial Transport Systems.- The Transport of Sugars Across Bacterial Membranes (Aharon-Katzir-Katchalsky Memorial Lecture).- Molecular Biology and Energetics of Membrane Transport.- Solubilisation and Partial Purification of Alanine Carrier from Membranes of a Thermophilic Bacterium and its Reconstitution into Functional Vesicles.- Some Ion Transport Systems in E. coli. (Transport of Potassium and of Anionic Sugars).- The Role of Periplasmic Proteins and Outer Membrane Receptors in the Process of Active Transport in E. coli.