Biochemical Actions of Hormones V14

Biochemical Actions of Hormones, Volume XIV is a compendium of papers dealing with DNA regulatory elements, leukotriene receptors, hormonal regulation of sodium-proton exchange activity, the structure of the mammalian progesterone receptor, stimulus-response, and signaling systems. One paper analyzes the hormonal factors that regulate the kinetic properties of the plasma membrane Na+ -H+ exchanger in epithelia and relates the control mechanisms to specific physiological function. Aqueous two-phase partitioning is a method that can be used in the study of steroid receptor activation and other conversions or interactions of steroid receptors. Using the two-phase partitioning, one can form a model of molecular changes during activation based on changes in the steroid receptor physico-chemical properties associated with the activation in vitro-phase. Another paper investigates the hypothesis that early androgenic modulation of nuclear effector-independent PKs is intimately related to the initial events in androgenic control of gene expression in the prostate. One paper describes the interaction between the estrogen receptor with DNA and the mechanism of transcription regulation by steroid receptors. Another paper reviews the role of GTP-binding proteins in receptor-mediated activation of phospholipase C, the formation and metabolism of novel inositol phosphates, as well as the possible role of protein kinase C in signal modulation. Micro-biologists, biochemists, molecular biologists, cellular biologists, and investigators in the field of cell research will find this collection useful.

Contenu

Preface

1. DNA Regulatory Elements for Steroid Hormone Receptors
   I. Introduction
   II. Hormone Regulatory Elements
   III. Mechanism of Transcriptional Regulation
   IV. Open Questions and Conclusions
   References
2. Signalling Systems in Stimulus-Response Coupling
   I. Introduction
   II. Receptor Coupling through GTP-Binding Proteins
   III. Metabolism of Inositol Lipids
   IV. Formation and Metabolism of Inositol Phosphates: Relationship to Ca2+ Release
   V. Feedback Control by Activation of Protein Kinase C
   VI. Summary
   References
3. Leukotriene Receptors and Signal Transduction Mechanism
   I. Introduction
   II. The Leukotriene Receptors
   III. Signal Transduction Systems
   IV. Conclusions
   References
4. Hormonal Regulation of Na+-H+ Exchange Activity in Epithelia
   I. Introduction
   II. Hormonal Effectors of Na+-H+ Exchange Activity
   III. The Physiological Consequences of Hormonal Regulation of Na+-H+ Exchange Activity
   IV. Independent and Dependent Effectors of Na+-H+ Exchange Activity
   V. Temporally Distinct Hormonal Effects on Na+-H+ Exchange Activity
   VI. Conclusions
   References
5. Aqueous Two-Phase Partitioning for the Study of Steroid Receptor Activation
   I. Introduction
   II. Methods Used to Study Steroid-Receptor Activation
   III. The Method of Aqueous Two-Phase Partitioning
   IV. Activation of Glucocorticoid Receptors and Estrogen Receptors Is Associated with a Change in Their Partition Coefficients
   V. Changes in Different Domains of the Glucocorticoid Receptor During Activation
   VI. Hormone-Dependent Changes in Receptor Properties
   VII. Kinetics of Glucocorticoid Receptor Activation
   VIII. Modifiers of Receptor Activation
   IX. Summary, Conclusions, and Perspectives
   References
6. Androgenic Regulation of Prostatic Protein Phosphokinases and Protein Phosphorylation
   I. Introduction
   II. The Experimental Model
   III. Prostatic Nuclear Protein Kinase Reactions
   IV. Prostatic Nuclear Protein Phosphokinases
   V. Prostatic Cytosolic Protein Kinases and Phosphoproteins
   VI. Factors That Influence Protein Kinase Reactions
   VII. Prostatic Nuclear-Associated Protein Phosphatases
   VIII. Age-Associated Changes in Prostatic Protein Kinases and Protein Phosphorylation
   IX. Concluding Comments
   References
7. Heat Shock Proteins and Steroid Hormone Receptors
   I. Introduction
   II. Composition of the Avian Progesterone Receptor
   III. Properties and Identification of the 90kDa Protein
   IV. Proteins Associated with Other Steroid Receptors
   V Possible Functions of hsp90
   References
8. The Allosteric Estrogen- and DNA-Binding Mechanism of the Estrogen Receptor
   I. Introduction
   II. The Site: Site Interactions of the Estrogen Receptor Induced by Steroid Binding
   III. Modulation of the Estrogen Receptor's Affinity for DNA by Estradiol
   IV Summary
   Reference
9. Structure of the Mammalian Progesterone Receptor
   I. Size of the Steroid Binding Subunit of the Progesterone Receptor
   II. Non-Steroid Binding Subunits of the Progesterone Receptor
   III. Receptor Phosphorylation
   IV. Receptor Gene and Messenger RNA
   References
   Index