Applied Bioengineering

Hochaktuelle und zukünftige Ausrichtungen von fünf Kernbereichen der Biotechnik, die für eine nachhaltige und innovative Produktion von unmittelbarer Bedeutung sind.

Auteur
Toshiomi Yoshida is Professor Emeritus of Osaka University (Japan). He received his doctorate degree at the same university in 1968, after studying fermentation technology. From 1972 to 1973, he visited the University of Pennsylvania (USA) for a research stay. In the further course of his career, he became Associate Professor at Osaka University in 1978, Full Professor in 1988 and held several positions within the university until his retirement in 2003. From 1995 to 1999, he was Director of the International Center for Biotechnology. After his retirement, Toshiomi Yoshida was appointed as Director of the Bangkok Liaison Office of the Japan Society for the Promotion of Science, a position he hold until 2007. From 2007 to 2012, he was Director General of the Research Institute of Environmental, Agriculture and Fisheries of the Osaka Prefectural Government. In between 2009 and 2013, Toshiomi Yoshida served as the first President of the Asian Federation of Biotechnology.

Résumé
A comprehensive overview of the topic, highlighting recent developments, ongoing research trends and future directions.
Experts from Europe, Asia and the US cover five core areas of imminent importance to the food, feed, pharmaceutical and water treatment industries in terms of sustainable and innovative processing and production. In the field of enzyme engineering, they summarize historic developments and provide an overview of molecular enzyme engineering, while also discussing key principles of microbial process engineering, including chapters on process development and control. Further sections deal with animal and plant cell culture engineering. The final section of the book deals with environmental topics and highlights the application of bioengineering principles in waste treatment and the recovery of valuable resources.
With its cutting-edge visions, extensive discussions and unique perspectives, this is a ready reference for biotechnologists, bioengineers, bioengineers, biotechnological institutes, and environmental chemists.

Contenu

List of Contributors XIX

1 Introduction 1
Toshiomi Yoshida

1.1 Introduction 1

1.2 Enzyme Technology 2

1.3 Microbial Process Engineering 2

1.4 Plant Cell Culture 5

1.5 Animal Cell Culture 5

1.6 Environmental Bioengineering 6

1.7 Composition of the Volume 7

References 7

Part I Enzyme Technology 11

2 Enzyme Technology: History and Current Trends 13
Klaus Buchholz and Uwe T. Bornscheuer

2.1 The Early Period up to 1890 13

2.2 The Period from 1890 to 1940 16

2.3 A New Biocatalyst Concept Immobilized Enzymes 19

2.4 Expanding Enzyme Application after the 1950s 24

2.5 Recombinant Technology A New Era in Biocatalysis and Enzyme Technology 27

2.6 Current Strategies for Biocatalyst Search and Tailor Design 32

2.7 Summary and Conclusions 39

Acknowledgment 40

Abbreviations 40

References 40

3 Molecular Engineering of Enzymes 47
Maria Elena Ortiz-Soto and Jürgen Seibel

3.1 Introduction 47

3.2 Protein Engineering: An Expanding Toolbox 48

3.3 High-Throughput Screening Systems 56

3.4 Engineered Enzymes for Improved Stability and Asymmetric Catalysis 58

3.5 De Novo Design of Catalysts: Novel Activities within Common Scaffolds 65

3.6 Conclusions 69

References 69

4 Biocatalytic Process Development 81
John M.Woodley

4.1 A Structured Approach to Biocatalytic Process Development 83

4.2 Process Metrics 83

4.3 Technologies for Implementation of Biocatalytic Processes 87

4.4 Industrial Development Examples 91

4.5 Future Outlook 95

4.6 Concluding Remarks 96

References 96

5 Development of Enzymatic Reactions in Miniaturized Reactors 99
Takeshi Honda, Hiroshi Yamaguchi, and Masaya Miyazaki

5.1 Introduction 99

5.2 Fundamental Techniques for Enzyme Immobilization 100

5.3 Novel Techniques for Enzyme Immobilization 150

5.4 Conclusions and Future Perspectives 155

Abbreviations 156

References 157

Part II Microbial Process Engineering 167

6 Bioreactor Development and Process Analytical Technology 169
Toshiomi Yoshida

6.1 Introduction 169

6.2 Bioreactor Development 170

6.3 Monitoring and Process Analytical Technology 196

6.4 Conclusion 203

Abbreviations 204

References 204

7 Omics-Integrated Approach for Metabolic State Analysis of Microbial Processes 213
Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa, Katsunori Yoshikawa, Yoshihiro Toya, Tomokazu Shirai, and Fumio Matsuda

7.1 General Introduction 213

7.2 Transcriptome Analysis of Microbial Status in Bioprocesses 214

7.3 Analysis of Metabolic State Based on Simulation in a Genome-Scale Model 219

7.4 13C-Based Metabolic Flux Analysis of Microbial Processes 223

7.5 Comprehensive Phenotypic Analysis of Genes Associated with Stress Tolerance 227

7.6 Multi-Omics Analysis and Data Integration 230

7.7 Future Aspects for Developing the Field 231

Acknowledgments 233

References 233

8 Control of Microbial Processes 237
Kazuyuki Shimizu, Hiroshi Shimizu, and Toshiomi Yoshida

8.1 Introduction 237

8.2 Monitoring 238

8.3 Bioprocess Control 242

8.4 Recent Trends in Monitoring and Control Technologies 250

8.5 Concluding Remarks 253

Abbreviations 254

References 254

Part III Plant Cell Culture and Engineering 259 9 Contained Molecular Farming Using Plant Cell and Tissue Cultures 261
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