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Molecular biotechnology continues to triumph, as this textbook testifies - edited by one of the academic pioneers in the field and written by experienced professionals. This completely revised second edition covers the entire spectrum, from the fundamentals of molecular and cell biology, via an overview of standard methods and technologies, the application of the various "-omics", and the development of novel drug targets, right up to the significance of system biology in biotechnology. The whole is rounded off by an introduction to industrial biotechnology as well as chapters on company foundation, patent law and marketing.
The new edition features:
Bonus material available online free of charge: www.wiley-vch.de/home/molecbiotech
Auteur
Michael Wink studied biology and chemistry in Bonn and was awarded his doctorate from TU Braunschweig in 1980. After gaining his lecturing qualification in 1984/1985, he was awarded a Heisenberg grant by the German Research Council to work at the Max Planck Institute for Breeding Research in Cologne and from then at the gene center of Ludwig-Maximilians University in Munich. Following a chair for pharmaceutical biology at Mainz University in 1988, he accepted the post of Professor for Pharmaceutical Biology at the University of Heidelberg one year later. Between 2002 and 2004 he was the founding and managing director of the Institute for Pharmacy and Molecular Biotechnology, and dean from 2001 to 2005 for the new and popular course in molecular biotechnology offered by the university. Professor Wink's areas of interest include pharmaceutical research, molecular biotechnology, and medicinal plants, as well as research into secondary materials and evolution.
Texte du rabat
Molecular biotechnology continues to triumph, as this textbook testifies edited by one of the academic pioneers in the field and written by experienced professionals. This updated, second edition covers the entire spectrum, from the fundamentals of molecular and cell biology, via an overview of standard methods and technologies, the application of the various -omics, and the development of novel drug targets, right up to the significance of system biology in biotechnology. The whole is rounded off by an introduction to industrial biotechnology as well as chapters on company foundation, patent law and marketing.
The new edition features:
• Large format and in color
• Clear-cut structure according to basics, methods, main topics and economic perspectives
• New sections on system biology, RNA interference, microscopic techniques, high throughput sequencing, laser applications, biocatalysis, current biomedical applications and drug approval
• Optimized teaching with learning targets, a glossary containing around 800 entries, over 500 important abbreviations and further reading.
The only resource for those who are seriously interested in the topic.
Bonus material available online free of charge:
www.wiley-vch.de/home/molecbiotech
Contenu
Preface XIX
List of Contributors XXI
Abbreviations XXV
Part I Fundamentals of Cellular and Molecular Biology 1
1 The Cell as the Basic Unit of Life 3
M. Wink
2 Structure and Function of Cellular Macromolecules 7
M. Wink
2.1 Structure and Function of Sugars 8
2.2 Structure of Membrane Lipids 10
2.3 Structure and Function of Proteins 14
2.4 Structure of Nucleotides and Nucleic Acids (DNA and RNA) 21
2.5 References 27
3 Structure and Functions of a Cell 29
M. Wink
3.1 Structure of a Eukaryotic Cell 29
3.2 Structure of Bacteria 50
3.3 Structure of Viruses 51
3.4 Differentiation of Cells 52
4 Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) 57
M. Wink
4.1 Genomes, Chromosomes, and Replication 57
4.2 Transcription: From Gene to Protein 71
4.3 Protein Biosynthesis (Translation) 76
5 Distributing Proteins in the Cell (Protein Sorting) 81
M. Wink
5.1 Import and Export of Proteins via the Nuclear Pore 82
5.2 Import of Proteins in Mitochondria and Chloroplasts 83
5.3 Protein Transport into the Endoplasmic Reticulum 85
5.4 Vesicle Transport from the ER via the Golgi Apparatus to the Cytoplasmic Membrane 86
6 Evolution and Diversity of Organisms 91
M. Wink
6.1 Prokaryotes 91
6.2 Eukaryotes 91
Part II Standard Methods in Molecular Biotechnology 99
7 Isolation and Purification of Proteins 101
T. Wieland, M. Lutz
7.1 Introduction 101
7.2 Producing a Protein Extract 102
7.3 Gel Electrophoretic Separation Methods 103
7.4 Methods of Protein Precipitation 106
7.5 Column Chromatography Methods 107
7.6 Examples 113
8 Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry 115
A. Schlosser, W.D. Lehmann
8.1 Introduction 115
8.2 Principles of Mass Spectrometry 115
8.3 Mass Precision, Resolution, and Isotope Distribution 116
8.4 Principles of ESI 116
8.5 Tandem Mass Spectrometers 117
8.6 Peptide Sequencing with MS/MS 119
8.7 Identifying Proteins with MS/MS Data and Protein Databases 120
8.8 Determining Protein Molecular Mass 121
8.9 Analysis of Covalent Protein Modification 122
8.10 Relative and Absolute Quantification 123
9 Isolation of DNA and RNA 125
H. Weiher, R. Zwacka, I. Herr
9.1 Introduction 125
9.2 DNA Isolation 125
9.3 RNA Isolation 127
10 Chromatography and Electrophoresis of Nucleic Acids 129
H. Weiher, R. Zwacka, I. Herr
10.1 Introduction 129
10.2 Chromatographic Separation of Nucleic Acids 129
10.3 Electrophoresis 130
11 Hybridization of Nucleic Acids 133
H. Weiher, R. Zwacka, I. Herr
11.1 Significance of Base Pairing 133
11.2 Experimental Hybridization: Kinetic and Thermodynamic Control 133
11.3 Analytical Techniques 134
12 Use of Enzymes in the Modification of Nucleic Acids 137
A. Groth, R. Zwacka, H. Weiher, I. Herr
12.1 Restriction Enzymes (Restriction Endonucleases) 137
12.2 Ligases 139
12.3 Methyltransferases 139
12.4 DNA Polymerases 140
12.5 RNA Polymerases and Reverse Transcriptase 141
12.6 Nucleases 141
12.7 T4 Polynucleotide Kinase 141
12.8 Phosphatases 142
13 Polymerase Chain Reaction 143
A. Mohr, H. Weiher, I. Herr, R. Zwacka