DNA and RNA Isolation Techniques for Non-Experts

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

Auteur
Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).

Contenu

1-Basic idea about nucleic acids for non-experts

2-General steps during isolation of DNA and RNA

3-Phenol-chloroform DNA isolation method

4-RNA isolation by the guanidinium-acid-phenol method

5-Spin column-based isolation of nucleic acid

6-Isolation of plasmid DNA by alkaline lysis

7-DNA Isolation by Hydrophilic Ionic Liquid Treatment

8-Lithium Chloride based isolation of DNA and RNA

9-Isolation of Bacteriophage DNA by PEG Method

10-DNA isolation by Chelex Method

11-DNA isolation by Lysozyme and Proteinase K

12-Salting-out method for blood samples

13-CTAB or SDS based isolation of plant's DNA

14-DNA extraction by spooling method

15-Magnetic bead-based nucleic acid isolation

16-Density gradient-based nucleic acid isolation

17-DNA extraction from agarose gel though paper-strips

18-TRANSFORMATION OR GENETIC MODIFICATION OF CELLS/ORGANISMS

19-GENE CLONING AND VECTORS

20-POLYMERASE CHAIN REACTION (PCR)

21-Southern and Northern Blotting

22-GENOME MAPPING

23-Applications of DNA Sequencing Technologies for Current Research