Advancements of Mass Spectrometry in Biomedical Research

The chapter "2D SDS PAGE in Combination with Western Blotting and Mass Spectrometry Is a Robust Method for Protein Analysis with Many Applications" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Auteur

Dr. Costel C. Darie started his biomedical career as a nurse at the Murgeni Hospital, Romania. He received his BS and MS in biochemistry at the Alexandru Ioan Cuza University from Iasi, Romania, and performed research under Dr. Vlad Artenie. He continued his Ph.D. studies with Dr. Wolfgang Haehnel at the Albert-Ludwigs Universitaet, Freiburg, Germany, after which he worked in the labs of Drs. Paul M. Wassarman & Micsunica Platica (Mount Sinai School of Medicine) and Thomas A. Neubert (New York University Medical Center). He currently is an Associate Professor of Chemistry and Biomolecular Science at Clarkson University, where he is the lead of the Biochemistry and Proteomics Group.

Dr. Alisa Woods received her BS in psychology from the State University of New York (SUNY) at Plattsburgh and her PhD in biology from the University of California, Irvine, with Dr. Christine Gall. She continued with post-doctoral training at the University of Freiburg, Germany, with Dr. Michael Frotscher and completed a Master's degree in Mental Health Counseling at the University of Massachusetts, Boston. She is currently a Research Associate Professor of Chemistry and Biomolecular Science at Clarkson University, where she works in the Laboratory for Biochemistry and Proteomics.

Résumé

This volume explores the use of mass spectrometry for biomedical applications. Chapters focus on specific therapeutic areas such as oncology, infectious disease, and psychiatry. Additional chapters focus on methodology, technologies and instrumentation, as well as on analysis of protein-protein interactions, protein quantitation, and protein post-translational modifications. Various omics fields such as proteomics, metabolomics, glycomics, lipidomics, and adductomics are also covered. Applications of mass spectrometry in biotechnological and pharmaceutical industry are also discussed. This volume provides readers with a comprehensive and informative manual that will allow them to appreciate mass spectrometry and proteomic research, but also to initiate and improve their own work. This book acts as a technical guide as well as a conceptual guide to the newest information in this exciting field.

Contenu

1.Mass Spectrometry for Proteomics-based Investigation

2.MALDI profiling and applications in medicine

3.Applications of Matrix-Assisted Laser Desorption Ionization In-Source Decay Mass Spectrometry

4.Proteome imaging: from classic to modern mass spectrometry-based molecular histology

5.Mass Spectrometry-Based Tissue Imaging of Small Molecules

6.Sequencing Proteins from Bottom to Top: Combining Techniques for Full Sequence Analysis of Glucokinase

7.Proteomics and non-proteomics approaches to study stable and transient protein-protein interactions

8.Contribution of Mass spectrometry-based Proteomics to Discoveries in Developmental Biology

9.Profiling optimal conditions for capturing EDEM proteins complexes in melanoma using mass spectrometry

10.The Methods employed in Mass Spectrometric Analysis of Posttranslational Modifications (PTMs) and ProteinProtein Interactions (PPIs)

11.Identification of post-translational modifications (PTMs) of proteins by mass spectrometry

12.Sequence Variant and Post-translational Modification Analysis During Cell Line Selection via High-throughput Peptide Mapping

13.Selective derivatization of hexahistidine-tagged recombinant proteins

14.Investigation of Antibody-Drug Conjugates by Mass Spectrometry

15.Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides

16.Strategies for Membrane Protein Analysis by Mass Spectrometry

17.The Potential for Ion Mobility in Pharmaceutical and Clinical Analyses

18.Application of Ion Mobility Mass Spectrometry in Lipidomics

19.Mass Spectrometry in Advancement of Redox Precision Medicine

20.Applications for mass spectrometry in the study of ion channel structure and function

21.Immuno affinity Mass Spectrometry: A Novel Approaches with Biomedical Relevance

22.Developing well-annotated species-specific protein databases using comparative proteogenomics

23.Stoichiometry of heavy metal binding to peptides involved in Alzheimer's disease: mass spectrometric evidence

24.Role of mass spectrometry in investigating a novel protein: the example of Tumor Differentiation Factor (TDF)

25.Mass Spectrometry-Based Biomarkers in Drug Developmen

26.Combinatorial Electrophoresis and Mass Spectrometry-Based Proteomics in Breast Milk for Breast Cancer Biomarker Discovery

27.Proteomic approaches to dissect neuronal signalling pathways

28.Mass Spectrometry for the Study of Autism and Neurodevelopmental Disorders

29.Simplifying the proteome: Analytical strategies for improving peak capacity

30.Exploration of nicotine metabolism in Paenarthrobacter nicotinovorans pAO1 by microbial proteomics

31.Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics

32.Mass spectrometry based comparative proteomics using one dimensional and two dimensional SDS-PAGE of rat atria induced with obstructive sleep apnea

33.2D SDS PAGE in combination with western blotting and mass spectrometry is a robust method for protein analysis with many applications.

34.Pulse SILAC approaches to the measurement of cellular dynamics

35.Protein Biomarkers in Major Depressive Disorder: an Update

36.A Pilot Exploratory Proteomics Investigation of Mental Fatigue and Mental Energy

37.Metabolomics and biomarker discovery

38.Analytical Methods for Mass Spectrometry-Based Metabolomics Studies

39.Trends in analysis of cortisol and its derivatives

40.Detection of biomedically relevant stilbenes from wines by mass spectrometry

41.Mass spectrometry as a complementary approach for noncovalently bound complexes based on cyclodextrins

42.Trends in glycolipid biomarker discovery in neurodegenerative disorders by mass spectrometry

43.Non-targeted Screening in Environmental Monitoring Programs

44.Mass Spectrometric DNA Adduct Quantification by Multiple Reaction Monitoring and its Future Use for the Molecular Epidemiology of Cancer

45.Bottlenecks in proteomics: an update

46.Recent Applications of Mass Spectrometry at Clarkson University

Index