Advanced Microscopy in Mycology

This volume provides insight into the principles underscoring various advanced microscopy methods and how they have been, or have the potential to be, applied to mycology. Offering a comprehensive overview of the confocal principle, confocal laser scanning microscopy and its application to fungal biology, this text also examines the newer sophisticated fluorescence-based methods of technology.

Auteur
Prof. Dr. Tanya Dahms serves as professor of Biochemistry and Microscopy at the University of Regina. She is head of the Dahms group, which currently studies microbial responses to external stimuli, including mechanisms associated with carbohydrate remodeling.

Résumé
The aim of this volume is to describe the latest advances in microscopic methods, including integrated techniques, as applied to mycology. Each chapter will provide a brief overview of a particular microscopic method with associated advantages and limitations, the research questions that can be appropriately addressed using these microscopic methods, how it has been successfully applied to address mycological research questions, including supporting and complimentary techniques, and which future questions can be addressed.

Contenu

Applications of Confocal Laser Scanning Microscopy in Filamentous Fungi.- Fluorescence-based Methods for the Study of Protein Localization, Interaction, and Dynamics in Filamentous Fungi.- Super Resolution Microscopy: SIM, STED and Localization Microscopy.- Fourier Transform Infrared (FTIR) Microscopy and Imaging of Fungi.- Whole Cells Imaged by Hard X-ray Transmission Microscopy.- In situ Nanocharacterization of Yeast Cells Using ESEM and FIB.- Imaging Living Yeasts Cells and Quantifying Their Biophysical Properties by Atomic Force Microscopy.- Future Directions in Mycological Microscopy.