A Practical Guide to Protein and Peptide Purification for Microsequencing

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

• Mass spectrophotometric characterization of proteins and peptides
• N-terminal sequencing of proteins with blocked N termini
• Internal amino acid sequence analysis after protease digestion in-gel and on-blot
• Improved microscale peptide purification methods
• Computer analysis of protein sequences
• New protocols tested and refined through everyday use in authors' laboratories
• Updated reference chapter covering all aspects of protein microsequencing

Contenu

Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation
Purification of Proteins and Peptides By SDS-PAGE
Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose
Mass Spectrometric Strategies for the Structural Characterization of Proteins