Discovery of Small-Molecule Modulators of Protein RNA Interactions for Treating Cancer and COVID-19

This book describes the development of novel proteinRNA-binding assays and their applications in a high-throughput manner for the identification of small-molecule modulators of proteinRNA interactions to treat cancer and COVID-19.

Modulating proteinRNA interactions with small molecules is expected to provide novel biological insights of the interrelation of diseases with the proteinRNA interactome. The modulations may also be exploited therapeutically. For these reasons, the development of a simple, reliable, and sensitive proteinRNA-binding assay is necessary for high-throughput screening to discover new effective chemical entities capable of acting on diverse proteinRNA interactions. This book discusses the discovery of small-molecule modulators targeting proteinRNA interactions that are potentially valuable to treat cancer and COVID-19 by constructing novel high-throughput screening methods. The results of this dissertation provide valuable insights into the regulation of proteinRNA interactions in chemical biology and drug development.

Discusses in detail the therapeutic significances of proteinRNA interaction modulators Highlights the development of proteinRNA-binding assays Examines in detail how proteinRNA interaction modulators can act as therapeutic agents

Auteur
Wan Gi Byun received his B.S. degree in Department of Chemistry at Seoul National University, Korea in 2015. In the same year, he joined Prof. Seung Bum Park's group in the Department of Chemistry at the Seoul National University and started to study chemical biology and drug discovery. His research during Ph.D. involved the development of high-throughput screening systems and identification of small-molecule modulators of protein-RNA interactions to overcome human diseases including cancer and COVID-19. After he got his Ph.D. degree in 2022, he is currently a post-doctoral researcher in Department of Chemistry, Seoul National University. His current research focus on the development of broadspectrum, pan-coronavirus antiviral drugs.

Texte du rabat

This book describes the development of novel protein RNA-binding assays and their applications in a high-throughput manner for the identification of small-molecule modulators of protein RNA interactions to treat cancer and COVID-19. Modulating protein RNA interactions with small molecules is expected to provide novel biological insights of the interrelation of diseases with the protein RNA interactome. The modulations may also be exploited therapeutically. For these reasons, the development of a simple, reliable, and sensitive protein RNA-binding assay is necessary for high-throughput screening to discover new effective chemical entities capable of acting on diverse protein RNA interactions. This book discusses the discovery of small-molecule modulators targeting protein RNA interactions that are potentially valuable to treat cancer and COVID-19 by constructing novel high-throughput screening methods. The results of this dissertation provide valuable insights into the regulation of protein RNA interactions in chemical biology and drug development.

Contenu

1. Introduction.- 2. Identification of Small-Molecule Inhibitors of Oncogenic Lin28Let-7 Interaction.- 3. Discovery of Small-Molecule Modulators of ProteinRNA Interactions by Fluorescence Intensity-Based Binding Assay.- 4. Harnessing Stress Granule Formation by Small Molecules to Inhibit the Cellular Replication of SARS-CoV-2.- Appendix.